The role and prognostic value of PANoptosis-related genes in skin cutaneous melanoma
Rattachement africain : cn, de. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Introduction: Skin cutaneous melanoma (SKCM), a malignant tumor, has PANoptosis implicated in its progression and metastasis. However, the exact mechanisms remain unclear. This study aims to develop a prognostic model for SKCM based on PANoptosis. Methods: SKCM - related datasets were retrieved from public databases. Differentially expressed PANoptosis - related genes (DEPRGs) were determined by intersecting differentially expressed genes from differential expression analysis and key module genes from weighted gene co - expression network analysis (WGCNA). Prognostic genes for SKCM were derived using Cox analysis and machine learning algorithms, leading to the construction and validation of a prognostic model. Independent prognostic factors were identified, and a nomogram was developed. Enrichment analysis and immune infiltration analysis were performed for the two risk groups. A competitive endogenous RNA (ceRNA) network was constructed, and potential therapeutic drugs were predicted. Bioinformatics findings were validated experimentally using reverse transcription quantitative PCR (RT - qPCR). Results: CD8A, ADAMDEC1, CD69, CRIP1, LSP1, BCL11B, and CCR7 were identified as prognostic genes. The risk model and nomogram showed excellent predictive abilities for SKCM patients. Genes in both high - and low - risk groups were linked to cytokine - regulated immune responses, with nine differential immune cells identified between the groups. The ceRNA network revealed that prognostic genes were regulated by several miRNAs (such as hsa-miR-330-5p) and lncRNAs (such as AL355075.4). MPPG and DT - 1687, associated with LSP1, may offer promising treatment options. RT - qPCR validation confirmed significant expression differences of CD8A, ADAMDEC1, CD69, CRIP1, and BCL11B between SKCM and control samples. Discussion: This study presents a robust prognostic model for SKCM based on PANoptosis - related genes, providing a theoretical foundation for SKCM treatment.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- The role and prognostic value of PANoptosis-related genes in skin cutaneous melanoma
- Date Crossref
- 06/06/2025
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Shanxi Medical University Shanxi Bethune Hospital pays non établi dans la noticeUniversité ou école supérieure
-
Shanxi Academy of Medical Sciences pays non établi dans la noticeÉtablissement de santé
-
Shanxi Provincial Cancer Hospital pays non établi dans la noticeÉtablissement de santé
-
Xian Central Hospital pays non établi dans la noticeÉtablissement de santé
-
University Hospital Bonn Institute of Molecular Medicine and Experimental Immunology (IMMEI) pays non établi dans la noticeÉtablissement de santé
-
Shanxi Province Cancer Hospital Department of General Medicine pays non établi dans la noticeÉtablissement de santé
-
Linfen Central Hospital Department of Thoracic Oncology pays non établi dans la noticeÉtablissement de santé
Shanxi Bethune Hospital — Shanxi Medical University, Shanxi Academy of Medical Sciences et Shanxi Provincial Cancer Hospital, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.