Full-length transcriptome provides insights into the molecular regulation of seed spike number in Agropyron mongolicum
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Le résumé fourni par la source
Agropyron mongolicum is a xerophytic perennial grass with good palatability and nutritional value, predominantly distributed in sandy, arid regions and desert steppes of northwest China. However, its low biomass and limited seed yield pose significant challenges for large-scale cultivation and forage production. To date, there have been limited genomic or transcriptomic studies on A. mongolicum, particularly regarding the molecular genetic mechanisms underlying yield-related traits. In this study, we employed an integrated transcriptomic approach combining long-read sequencing (PacBio Iso-Seq) and short-read sequencing (Illumina RNA-seq) to generate a comprehensive full-length transcriptome from four tissues—leaves, stems, young spikes, and mature spikes. Transcriptomic comparisons between multi-spike and few-spike germplasms were performed to identify candidate genes involved in the regulation of spike number. A total of 762,116 full-length transcripts were generated, among which 214,500 were successfully annotated. We identified 185,424 unique genes, including 91,514 differentially expressed genes (DEGs). Functional enrichment analysis revealed 43 DEGs associated with the protein processing pathway in the endoplasmic reticulum. Notably, 8 DEGs were specifically expressed in young spikes and mature spikes of the contrasting spike-number germplasms, suggesting their potential role in spike number regulation. This study provides a high-quality transcriptomic resource and identifies candidate genes that may facilitate molecular breeding for enhanced seed yield in A. mongolicum.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Full-length transcriptome provides insights into the molecular regulation of seed spike number in Agropyron mongolicum
- Date Crossref
- 03/06/2025
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Inner Mongolia Agricultural University pays non établi dans la noticeUniversité ou école supérieure
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State Forestry and Grassland Administration pays non établi dans la noticeOrganisme public
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Institute of Grassland Research pays non établi dans la noticeStructure de recherche
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Inner Mongolia Electric Power (China) pays non établi dans la noticeEntreprise
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Inner Mongolia Electric Power Survey & Design Institute (China) pays non établi dans la noticeEntreprise
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Inner Mongolia University of Technology pays non établi dans la noticeUniversité ou école supérieure
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College of Grassland Science pays non établi dans la noticeUniversité ou école supérieure
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Institute of Ecological Protection and Restoration pays non établi dans la noticeStructure de recherche
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Research Department of Inner Mongolia Grassland Technology Innovation Center Co. pays non établi dans la noticeOrganisation à but non lucratif
Inner Mongolia Agricultural University, State Forestry and Grassland Administration et Institute of Grassland Research, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.