Constructing Built-In Electric Field in Hierarchical-Flower Heterostructure for High-Performance Serum Metabolic Assay
Rattachement africain : cn. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Laser desorption ionization mass spectrometry (LDI-MS) is a critical platform for high-throughput nontargeted metabolomics analysis in clinical diagnosis. However, traditional organic matrices inherently suffer from background interference in the low-mass range and exhibit low sensitivity for small molecule detections. Heterostructure has been regarded as an effective structure for high charge carrier mobility and tunable band gaps, which can enhance ion transfer efficiency and photothermal conversion during the LDI-MS process. In this work, Fe 3 O 4 /MoS 2 nanoparticles with hierarchical-flower heterostructure were facilely synthesized as a novel matrix of LDI-MS to enhance the detection of serum metabolic profilings (SMPs), which was further applied for the early diagnosis of lung cancer. The heterostructure of Fe 3 O 4 /MoS 2 can construct a built-in electric field to inhibit electron–hole recombination. Additionally, its abundant defect structures synergistically accelerate interfacial charge transfer, thereby promoting desorption and ionization processes. As a result, the newly developed Fe 3 O 4 /MoS 2 nanomatrix demonstrated exceptional performance in LDI-MS, significantly surpassing the conventional matrices by at least 1 order of magnitude. Subsequently, information-rich SMPs were successfully obtained from merely 1 μL of serum. More than 90% of the metabolic features exhibited RSDs below 30% in quality control samples, highlighting the high reproducibility of our method for clinical applications. Furthermore, hundreds of lung cancer patients and healthy controls can be clearly distinguished based on their SMPs by using appropriate machine learning models. Finally, two key metabolites associated with lung cancer were identified as potential biomarkers, which showed promising diagnostic capability with an AUC value of 0.824 in the validation set. Taken together, Fe 3 O 4 /MoS 2 nanoparticles emerge as a promising nanomatrix with superior LDI efficiency and the developed LDI-MS platform proves to be a powerful tool for serum metabolic profiling, offering significant potential for lung cancer diagnosis.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Constructing Built-In Electric Field in Hierarchical-Flower Heterostructure for High-Performance Serum Metabolic Assay
- Date Crossref
- 20/05/2025
- Éditeur
- American Chemical Society (ACS)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Dalian Institute of Chemical Physics pays non établi dans la noticeStructure de recherche
-
Chinese Academy of Sciences pays non établi dans la noticeOrganisme public
-
Beijing Proteome Research Center pays non établi dans la noticeStructure de recherche
-
University of Chinese Academy of Sciences pays non établi dans la noticeUniversité ou école supérieure
-
Dalian Medical University pays non établi dans la noticeUniversité ou école supérieure
-
First Affiliated Hospital of Dalian Medical University pays non établi dans la noticeÉtablissement de santé
-
Liaoning Province Key Laboratory of Metabolomics pays non établi dans la noticeStructure de recherche
-
State Key Laboratory of Medical Proteomics pays non établi dans la noticeStructure de recherche
Dalian Institute of Chemical Physics, Chinese Academy of Sciences et Beijing Proteome Research Center, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.