Bronchoalveolar Lavage Fluid Cell Gene Expression Signatures Are Suggestive of Prolonged Innate Immune Activation in Patients With Persistent Post-covid-19 ILD
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Le résumé fourni par la source
Abstract Rationale/Aim: Survivors of COVID-19 can experience long-term lung complications (pulmonary sequelae), but the underlying mechanisms remain unclear. Our study aimed to analyse the bronchoalveolar lavage fluid (BALF) cell compartment of patients with post-COVID-19 ILD and address the question why some patients despite presenting with extensive ILD a few months following COVID-19, make a full recovery after a year, while others display persistent ILD.Methods: To investigate this, BALF from a group of patients with post-COVID-19 ILD six months after acute COVID-19, was analysed. Patients were categorized into two groups, based on HRCT evaluation one year later: those with persistent HRCT abnormalities compatible with fibrosis (persistent post-COVID-19 ILD, n=6) and those with resolved lung lesions (resolved post-COVID-19 ILD, n=13). Additionally, 6 patients with pre-existing ILD after COVID-19 recovery, were included in the study.Results: Bulk RNA sequencing data from BALF cells revealed enriched pathways associated with neutrophil and monocyte chemotaxis in patients with persistent HRCT abnormalities, consistent with increased monocyte-like cell percentages in the BALF. Furthermore, the expression levels of markers of pro-fibrotic macrophages such as SPP1, surfactant proteins and pro-inflammatory cytokines were also increased. Conversely, patients with resolved post-COVID-19 ILD, displayed BALF cell gene expression signatures indicative of adaptive immune response and T-cell activation. BALF gene expression patterns associated with pro-fibrotic macrophage activation, neutrophil chemotaxis and downregulation of T-cell activation were also observed in patients with pre-existing fibrotic ILD following COVID-19 in comparison to the resolved post-COVID-19 ILD group.Conclusion: Our findings suggest that immune response imbalance due to prolonged activation of innate immunity and inadequate adaptive immune responses are associated with persistent post-COVID-19 ILD.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Bronchoalveolar Lavage Fluid Cell Gene Expression Signatures Are Suggestive of Prolonged Innate Immune Activation in Patients With Persistent Post-covid-19 ILD
- Date Crossref
- 01/05/2025
- Éditeur
- Oxford University Press (OUP)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Crete pays non établi dans la noticeUniversité ou école supérieure
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Alexander Fleming Biomedical Sciences Research Center pays non établi dans la noticeStructure de recherche
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University Hospital of Heraklion Department of Radiology pays non établi dans la noticeÉtablissement de santé
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St Thomas' Hospital pays non établi dans la noticeÉtablissement de santé
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Royal Brompton Hospital Interstitial lung disease Unit pays non établi dans la noticeÉtablissement de santé
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School of Medicine Department of Respiratory Medicine pays non établi dans la noticeUniversité ou école supérieure
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Institute for Fundamental Biomedical Research pays non établi dans la noticeStructure de recherche
University of Crete, Alexander Fleming Biomedical Sciences Research Center et Department of Radiology — University Hospital of Heraklion, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.