Effect of the Supplementation of L-Aspartic Acid, L-Ascorbic Acid, and Silver Nanoparticles (AgNPs) to the In vitro Fertilization (IVF) Media on the NMRI Mouse IVF Rates
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Le résumé fourni par la source
Background: Numerous lifestyle factors contribute to the incidence of infertility, which is a major concern for couples throughout the globe. The effectiveness of in vitro fertilization (IVF) is highly dependent on the characteristics of the culture media used. Objective: The present research study aimed to determine the effects of L-aspartic acid, L-ascorbic acid, and silver nanoparticles (AgNPs) on the success rate of IVF in NMRI mice. Methods: The solutions of L-aspartic acid, L-ascorbic acid, and synthesized AgNPs were prepared. The 6-8 weeks-old NMRI male mice were used as sperm donors, and 6-8 weeks-old NMRI mice were used for oocyte donation. In addition, the IVF procedure was performed using 50 μL drops of KSOM+5%BSA in mineral oil. Seven different experimental groups were investigated in addition to the control group. Oocytes were exposed to sperm for 4-6 h in the KSOM medium as a control group. Results: According to the results, the groups containing supplements "AgNPs", "L-aspartic acid", and "AgNPs + L-ascorbic acid", were not suitable for the 2PN stage. In contrast, the group containing "L-aspartic acid + L-ascorbic acid" was the best group for the 2PN stage. In addition, supplementation of the mixture of L-aspartic acid and AgNPs solution in KSOM+BSA culture medium was the most suitable group for the 2Cell stage. Conclusion: In conclusion, the study highlighted the varying impacts of L-aspartic acid, L-ascorbic acid, and AgNPs on IVF outcomes in NMRI mice. Group E, combining L-aspartic acid and AgNPs, emerged as effective for 2Cell development. Future research should explore the underlying biochemical mechanisms of these findings. Furthermore, another study is in progress that utilizes these supplements to generate 2PN first, and then the supplement-free medium as the cleavage media.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Effect of the Supplementation of L-Aspartic Acid, L-Ascorbic Acid, and Silver Nanoparticles (AgNPs) to the In vitro Fertilization (IVF) Media on the NMRI Mouse IVF Rates
- Date Crossref
- 01/01/2026
- Éditeur
- Bentham Science Publishers Ltd.
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Islamic Azad University Department ofBiology pays non établi dans la noticeUniversité ou école supérieure
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Royan Institute pays non établi dans la noticeOrganisation à but non lucratif
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Tehran University of Medical Sciences pays non établi dans la noticeUniversité ou école supérieure
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Zanjan University of Medical Sciences pays non établi dans la noticeUniversité ou école supérieure
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University of Tehran pays non établi dans la noticeUniversité ou école supérieure
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Gonabad University of Medical Sciences pays non établi dans la noticeUniversité ou école supérieure
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Reproductive Biomedicine Research Center Department of Andrology pays non établi dans la noticeStructure de recherche
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School ofMedicine Department of Anatomy pays non établi dans la noticeUniversité ou école supérieure
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School of Medicine Department of Anatomy pays non établi dans la noticeUniversité ou école supérieure
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School of Mechanical Engineering pays non établi dans la noticeUniversité ou école supérieure
Department ofBiology — Islamic Azad University, Royan Institute et Tehran University of Medical Sciences, avec 7 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.