MicroRNA-665 and its potential role in drug response and survival outcomes in multiple myeloma: a preliminary study
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Le résumé fourni par la source
Background Multiple myeloma (MM) is a complex hematological malignancy with heterogeneous clinical and pathophysiological backgrounds that influence treatment responses and outcomes. Identifying biomarkers to predict drug response and guide treatment decisions, particularly regarding drug combinations, is essential to improve therapeutic efficacy and patient outcomes. This study explores the role of microRNAs (miRNAs/miRs) derived from bone marrow (BM) and peripheral blood (PB) in responses to treatment and survival outcomes in newly diagnosed MM (ndMM) patients. Methods This study included twenty patients with ndMM undergoing first-line treatment with bortezomib, thalidomide, and dexamethasone. The miRNAs were isolated from BM and PB, and their profiles were analyzed using Next-Generation Sequencing (NGS), followed by validation of differentially expressed miRNAs by quantitative real-time PCR (qPCR). Clinical and response data were collected to assess correlations between miRNA levels, clinical characteristics, and patient outcomes. In silico analysis for target-prediction and gene ontology (GO) enrichment was performed to explore the potential biological and functional role of the identified miRNAs. Results NGS profiling revealed several miRNAs differently expressed between treatment-refractory and sensitive patients, as well as between PB and BM. Among these, miR-665, miR-483-5p, miR-143-3p and miR-145-5p were selected for further validation by qPCR. It was observed that miR-665 was significantly elevated in treatment-refractory patients compared to treatment-sensitive patients. Additionally, miR-665 levels were higher in PB than in BM. Elevated miR-665 levels were associated with more aggressive disease characteristics and poorer clinical outcomes, including reduced overall survival. Discussion Our preliminary findings suggest that miR-665 could potentially serve as a non-invasive tool for predicting drug resistance and guiding treatment decisions in MM. These findings also highlight the potential utility of miRNAs in liquid biopsies as a predictive tool of drug response in MM and could pave the way for personalized treatment strategies, improving patient outcomes. Future research is needed to validate these results in larger cohorts and explore the underlying mechanisms of miR-665 in MM pathogenesis and drug resistance.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- MicroRNA-665 and its potential role in drug response and survival outcomes in multiple myeloma: a preliminary study
- Date Crossref
- 04/04/2025
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Universidade do Porto pays non établi dans la noticeUniversité ou école supérieure
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Hospital de São João pays non établi dans la noticeÉtablissement de santé
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Universidade Católica Portuguesa pays non établi dans la noticeUniversité ou école supérieure
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Universidade Nova de Lisboa FCM pays non établi dans la noticeUniversité ou école supérieure
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Cooperativa de Ensino Superior Politécnico e Universitário pays non établi dans la noticeUniversité ou école supérieure
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FMUP - Faculty of Medicine of the University of Porto Clinical Hematology pays non établi dans la noticeUniversité ou école supérieure
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Clinical Hematology pays non établi dans la noticeÉtablissement de santé
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IPATIMUP - Institute of Molecular Pathology and Immunology of the University of Porto pays non établi dans la noticeUniversité ou école supérieure
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FFUP - Faculty of Pharmacy of the University of Porto Department of Biological Sciences pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Dental Medicine (FMD) pays non établi dans la noticeUniversité ou école supérieure
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Laboratory of Integrative and Translocation Research in Population Health (ITR) pays non établi dans la noticeStructure de recherche
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School of Medicine and Biomedical Sciences (ICBAS) Unit for Multidisciplinary Research in Biomedicine (UMIB) pays non établi dans la noticeUniversité ou école supérieure
Universidade do Porto, Hospital de São João et Universidade Católica Portuguesa, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.