Metagenome Processing in Clinical Setting for Respiratory Pathogens v1
Rattachement africain : Afrique du Sud, in. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Metagenomic bioinformatics pipelines are essential for extracting meaningful biological insights from the vast amounts of sequencing data generated in microbial studies. However, current approaches face several challenges that limit their utility and accessibility. A major hurdle is the efficient and accurate removal of human DNA reads from metagenomic datasets, a critical step for improving the sensitivity of pathogen detection. This process often demands significant computational resources and can become a bottleneck in large-scale analyses. In addition, most existing pipelines are designed to focus on specific microbial groups, making it difficult to comprehensively analyse the full spectrum of microbial communities, including bacteria and viruses, from a single sample. This lack of unified, end-to-end solutions limits both the scope and reproducibility of metagenomic research. To address these limitations, we developed a comprehensive protocol that streamlines host DNA removal and enables the simultaneous profiling of diverse microbial taxa from a single FASTQ file. This workflow consolidates existing pipelines within a unified framework, optimising computational efficiency while producing comprehensive microbial profiles. Our approach bridges a critical gap in metagenomics by supporting both clinical and environmental research needs in a scalable and conscious manner.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Metagenome Processing in Clinical Setting for Respiratory Pathogens v1
- Date Crossref
- 19/03/2025
- Éditeur
- Springer Science and Business Media LLC
- Type
- posted-content
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Western Cape Department of Health Western Cape Department of Health, Afrique du Sud (code pays fourni par la source)Organisme public
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National Institute of Research in Tuberculosis pays non établi dans la noticeStructure de recherche
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DSI NRF Centre of Excellence for Biomedical Tuberculosis Research pays non établi dans la noticeInstitution
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Faculty of Medicine and Health Sciences Division of Molecular Biology and Human Genetics Stellenbosch University, Afrique du Sud (pays nommé en fin d’affiliation)Université ou école supérieure
Western Cape Department of Health (Western Cape Department of Health, Afrique du Sud), National Institute of Research in Tuberculosis et DSI NRF Centre of Excellence for Biomedical Tuberculosis Research, avec 1 autre affiliation. Pays d’affiliation : Afrique du Sud.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.