Author Correction: KN3014, a piperidine-containing small compound, inhibits auto-secretion of IL-1β from PBMCs in a patient with Muckle–Wells syndrome

Le résumé fourni par la source

Due to an error during figure assembly Figure 3e, panel “Vehicle/LPS(-)” duplicates Figure 3c, panel “MCC950 (50 μM)/LPS (0.1 ng/mL)”. The corrected version of Figure 3 is provided below. Fig. 3 KN3014 reduced ASC-speck formation accompanied by IL-1β processing and secretion with no effect on pro-IL-1β production or NF-κB activation. ( a ) Western blotting analysis of LPS (0.1 ng/mL) induced pro-IL-1β production (upper panel) and IL-1β cleavage (middle panel; the same blotting membrane shown in the upper panel was re-hybridized with a mAb specific for cleaved-IL-1β (long exposure)). The same gel was stained with Coomassie brilliant blue (lower panel). ( b ) IL-1β concentrations in the supernatant of each well measured by ELISA. ( c ) Immunofluorescence microscopy evaluation of ASC-speck formation. ( d ) Percentage of ASC specks (%). ( e ) Immunofluorescence microscopy evaluation of NF-κB p65 nuclear translocation. ( f ) Relative nuclear translocation of NF-κB p65. Results are given as means ± standard deviation of three hyper views. *p-value < 0.05 (Mann–Whitney U -test). These results are representative of two independent experiments. Scale bars = 10 μm. Full size image

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