Real‐Time PCR Helicobacter pylori Test in Comparison With Culture and Histology for Helicobacter pylori Detection and Identification of Resistance to Clarithromycin: A Single‐Center Real‐Life Study
Rattachement africain : fr. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
BACKGROUND: In our center, RT-PCR was integrated as a routine method to diagnose Helicobacter pylori due to its higher availability after COVID-19 pandemics. The objective of this study was to assess the feasibility and performance of systematically performed RT-PCR for H. pylori detection and clarithromycin (CLA) resistance in a real-life clinical practice. MATERIALS AND METHODS: One hundred consecutive patients underwent an upper digestive endoscopy during which at least four biopsies (two from the antrum and two from the corpus) were obtained for RT-PCR and culture with antibiogram and four additional biopsies for histology. The results of H. pylori detection were compared among RT-PCR, histology, and bacterial culture, and the results of CLA susceptibility were compared between culture-based antibiogram and RT-PCR. RESULTS: Out of 100 patients, 64 were positive for H. pylori by RT-PCR, 66 by histology, and 53 by culture, with no statistically significant difference among the three methods (p > 0.05). CLA resistance was found in 8 out of 45 patients (17.7%) by culture and in 12 out of 64 patients (18.7%) by PCR. In 8 H. pylori-positive patients by culture, the antibiogram could not be realized due to lack of viability of the strains. In one patient, after a double checking, discrepant results were observed, requiring a complementary molecular analysis by the French National Reference Center for Helicobacters, which confirmed the existence of a double population of H. pylori strains within biopsies, with and without CLA resistance. CONCLUSIONS: Our study demonstrates that in real-life clinical practice, RT-PCR is feasible and comparable in the ability to detect H. pylori and its resistance to CLA to bacterial culture with antibiogram and histology. Given its rapidity and limited dependence on the operator's interpretation, it appears preferable to the other methods.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Real‐Time <scp>PCR</scp><i>Helicobacter pylori</i> Test in Comparison With Culture and Histology for <i>Helicobacter pylori</i> Detection and Identification of Resistance to Clarithromycin: A Single‐Center Real‐Life Study
- Date Crossref
- 01/03/2025
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Centre Hospitalier Universitaire de Nantes pays non établi dans la noticeÉtablissement de santé
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Nantes Université INCIT pays non établi dans la noticeUniversité ou école supérieure
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Université de Bordeaux pays non établi dans la noticeUniversité ou école supérieure
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Inserm pays non établi dans la noticeOrganisme public
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Immunologie et Nouveaux Concepts en Immunothérapie pays non établi dans la noticeStructure de recherche
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Department of Bacteriology and Microbiological Control University Hospital of Nantes Nantes France pays non établi dans la noticeUniversité ou école supérieure
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Department of Pathology University Hospital of Nantes Nantes France pays non établi dans la noticeUniversité ou école supérieure
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University of Bordeaux Bordeaux France INSERM U1312 BRIC pays non établi dans la noticeUniversité ou école supérieure
Centre Hospitalier Universitaire de Nantes, INCIT — Nantes Université et Université de Bordeaux, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.