Development and Validation of a Sensitive and Accurate LC-MS/MS Method for the Quantification of Creatinine in Rat Urine

Résumé fourni par la source

Creatinine which is produced during muscle energy production serves as an indirect marker of kidney function in clinical chemistry and as a parameter for urine matrix integrity in forensic toxicology.Urinary creatinine concentration is also used as a normalization factor for individual differences in drugs and metabolites in bioanalysis.Although liquid chromatography-tandem mass spectrometry (LC-MS/MS) has shown considerable potential for the quantification of creatinine in urine samples, but sufficient validation has not been performed.Therefore, herein, we aimed to develop and validate a sensitive and accurate quantitative method for measuring creatinine levels in rat urine using LC-MS/MS.Water was used as a surrogate matrix for rat urine, and its suitability was fully demonstrated.The validation results demonstrated that the method was selective, sensitive, accurate, and precise, with acceptable linearity within a defined calibration range.The limit of detection and lower limit of quantification were 5.0 and 50 μg/mL, respectively.In the urine samples of healthy rats (n = 6), the concentrations of creatinine, as determined via the developed method, ranged from 116.1 to 445.8 μg/mL.This method is expected to be highly beneficial for quantitatively determining the levels of drugs and metabolites in rat urine.

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