In vitro propagation of commercial rose variety using nodal explant culture

Le résumé fourni par la source

The clonal propagation strategy of plant tissue culture can produce a large quantity of high-quality planting material in a relatively short time. The study focuses on mass propagation of roses using tissue culture techniques. The method is suitable for rose varieties with high commercial value, such as cut flowers and potted plants. Standardization of surface sterilization techniques involved treatment of explants with fungicides, antibiotics, and mercuric chloride solutions. We found that antibiotic treatment was essential to remove the bacterial infection, which could be due to endophytic bacteria in the plant material. For in vitro shoot regeneration, MS medium supplemented with BAP worked best, followed by medium supplemented with a mix of cytokinins. The in vitro regenerated shoots were then multiplied on the medium supplemented with BAP with periodic subculturing to maintain shoot cultures, which can be subsequently rooted and transplanted onto the field.

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