Investigation of the effects of chrysin on intestinal ischemia-reperfusion via apoptotic, oxidative stress, inflammation, and autophagy parameters

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Aim: This study aimed to investigate chrysin's molecular, biochemical, and histological effects in an experimental intestinal ischemia/reperfusion (IR) model and to reveal possible protective mechanisms. Material-Methods: 35 Wistar rats were randomly divided into five groups: Control, CHR, IR, IR+CHR25, IR+CHR50. The IR model was established by inducing ischemia by ligating the superior mesenteric artery for one hour and restoring blood flow for two hours. In the study, MDA and GSH levels were analysed by manual biochemical method; SOD, CAT, GPx activities and NF-κB and NO levels by ELISA method; caspase-3, Beclin-1, LC3A, PERK, ATF-6 mRNA transcription levels by RT-PCR method. In addition, tissue structure was examined histologically. Results: MDA levels were doubled in the IR group and decreased with CHR (p< .05). In addition, CHR increased SOD, CAT, and GPx activities and GSH levels which decreased due to IR (p< .05). Inflammation markers NF-κB and NO were increased; and decreased with CHR (p< .05). Apoptosis marker caspase-3 increased in IR and decreased with CHR (p< .05). Autophagy markers Beclin-1 and LC3A were increased by CHR (p< .05); endoplasmic reticulum stress markers PERK and ATF-6 were increased in IR and decreased by CHR (p< .05). Severe histopathologic changes were observed in the IR and improved with CHR treatment. Conclusion: While IR causes damage to intestinal tissue, the antioxidant and anti-inflammatory properties of CHR have revealed its therapeutic potential against IR injury.

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