Transcriptomic and proteomic-based analysis of the mechanisms by which drought and salt stresses affect the quality of Isatidis Folium
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Le résumé fourni par la source
Isatidis Folium, derived from the dried leaves of Isatis indigotica Fort., has been used for centuries as a traditional Chinese herb with antibacterial and antiviral properties. However, heterogeneity in cultivation conditions and climatic variations poses challenges to accurately and effectively evaluate its quality. Current quality control methods cannot provide a comprehensive and effective identification of herbal quality and preparation efficacy. This study aimed to investigate the impact of different environmental factors on the biosynthesis and accumulation of medicinal components and identify biomarker genes and functional proteins associated with abiotic stress responses of Isatis indigotica Fort. We proposed evaluating the quality of Isatidis Folium based on multi-component quantitative analysis and integrating transcriptomic, proteomic, and physiological indicators to elucidate the mechanisms of herbal quality variation. The results revealed that abiotic stress conditions significantly altered the levels of bioactive constituents, physiological indices, and specific genes and proteins. Notably, biological pathways such as porphyrin metabolism, photosynthesis, and carbon fixation by photosynthetic organisms were implicated in phototoxicity within the photosystem under abiotic stresses. Biological pathways related to indole metabolism, specifically phenylalanine, tyrosine, and tryptophan synthesis, tryptophan metabolism, and indole alkaloid synthesis, were recognized as critical regulatory networks modulating indole alkaloid content. Candidate biomarkers such as HemB, PsbB, RBS2, RIBA2, TRPA, and TRPB were identified as potential factors of quality deterioration under adverse conditions. Based on the integration of chemical analysis and multi-omics techniques, a new hierarchical quality control scenario for Isatidis Folium was finally proposed, providing a research foundation for the innovation-driven development of traditional Chinese medicine.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Transcriptomic and proteomic-based analysis of the mechanisms by which drought and salt stresses affect the quality of Isatidis Folium
- Date Crossref
- 14/03/2025
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Shandong Provincial QianFoShan Hospital Department of Clinical Pharmacy pays non établi dans la noticeÉtablissement de santé
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Shandong First Medical University pays non établi dans la noticeUniversité ou école supérieure
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Shandong University of Traditional Chinese Medicine pays non établi dans la noticeUniversité ou école supérieure
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Shandong Academy of Chinese Medicine pays non établi dans la noticeStructure de recherche
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Biomedical Sciences College & Shandong Medical Biotechnology Research Center pays non établi dans la noticeUniversité ou école supérieure
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College of Traditional Chinese Medicine pays non établi dans la noticeUniversité ou école supérieure
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Institute of Traditional Chinese Medicine Pharmacology pays non établi dans la noticeStructure de recherche
Department of Clinical Pharmacy — Shandong Provincial QianFoShan Hospital, Shandong First Medical University et Shandong University of Traditional Chinese Medicine, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.