Green synthesis of copper oxide nanoparticles from Algerian propolis: Exploring biochemical, structural, antimicrobial, and anti-diabetic properties

Résumé fourni par la source

Abstract The green synthesis of nanoparticles (NPs) using natural extracts offers an eco-friendly alternative to traditional methods. In this study, we synthesized copper oxide nanoparticles (CuO NPs) using propolis extract as a natural reducing agent, resulting in two variants: CuO A and CuO B (calcined). UV-Vis spectroscopy confirmed successful synthesis, revealing distinct optical properties influenced by thermal treatment. Fourier transform infrared (FTIR) spectroscopy was performed to identify bioactive compounds stabilizing the NPs, with Cu–O stretching bands at 603 cm⁻¹ for CuO A and at 633.6, 596.4, and 484.6 cm⁻¹ for CuO B. X-ray diffraction determined crystallite sizes of 68.5 nm (CuO A) and 74.82 nm (CuO B). Scanning electron microscopy showed spherical shapes for CuO A and star-shaped forms for CuO B. Biological assays revealed superior antioxidant activity for CuO A (IC 50 = 0.027, AEAC = 2.01) compared to CuO B (IC 50 = 0.052, AEAC = 1.76). CuO A also demonstrated higher total antioxidant capacity (TAC = 11.28 mg EAA/g NPs) and antimicrobial efficacy, with lower minimum inhibitory concentration (MIC = 5–10 mg·mL −1 ) than CuO B (MIC = 20–80 mg·mL −1 ). Its enhanced glucose absorption capacity highlights its potential antidiabetic applications. These findings underscore the superior biological properties of CuO A, demonstrating its promising biomedical potential.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.