Toxicity and absorption of polystyrene micro-nanoplastics in healthy and Crohn’s disease human duodenum-chip models
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Le résumé fourni par la source
Micro and nanoplastics (MNPs) are widespread environmental and food web contaminants that are absorbed by the intestine and distributed systemically, but the mechanisms of uptake are not well understood. In a triculture small intestinal epithelial model, we previously found that uptake of 26 nm polystyrene MNPs (PS26) occurred by both passive diffusion and active actin- and dynamin-dependent mechanisms. However, studies in a more physiologically relevant model are required to validate those results. Here, a microfluidic intestine-on-a-chip model was developed using primary human intestinal epithelial organoids from healthy and Crohn’s disease donors, and used to evaluate the toxicity and mechanisms effectuating uptake of 25 nm polystyrene shell-gold core tracer MNPs (AuPS25). AuPS25 caused minimal toxicity after 24 h exposure in either healthy or Crohn’s IOC models. RNAseq analysis of epithelial cells identified 9 genes dysregulated by AuPS25, including downregulation of IFI6 (interferon alpha-induced protein 6). Because IFI6 has important antiviral and immunosuppressive functions in the intestine, its downregulation suggests impairment of innate immune function, which could have important negative health consequences. Inhibitor studies revealed that AuPS25 uptake in the IOC occurred by both passive diffusion and active actin- and dynamin-dependent mechanisms, consistent with our previous findings in the triculture model. • Human organoid and primary endothelial cell-based intestine-on-a chip (IOC) model. • Toxicity and uptake of Au-core 25 nm polystyrene (AuPS25) tracer particles in IOC. • AuPS25 Uptake by passive and active actin- and dynamin-dependent mechanisms. • AuPS25 caused no toxicity in heathy or Crohn’s disease IOC at 60 µg/mL for 24 h. • AuPS25 downregulated IFI6 (immunosuppressive and antiviral functions) expression.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Toxicity and absorption of polystyrene micro-nanoplastics in healthy and Crohn’s disease human duodenum-chip models
- Date Crossref
- 01/06/2025
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Environmental and Occupational Health Sciences Institute Nanoscience and Advanced Materials Center pays non établi dans la noticeUniversité ou école supérieure
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The State University of New Jersey Rutgers pays non établi dans la noticeUniversité ou école supérieure
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Harvard University Wyss Institute for Biologically Inspired Engineering pays non établi dans la noticeUniversité ou école supérieure
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Connecticut Agricultural Experiment Station pays non établi dans la noticeOrganisme public
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Rutgers University Department of Genetics pays non établi dans la noticeUniversité ou école supérieure
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Department of Analytical Chemistry pays non établi dans la noticeInstitution
Nanoscience and Advanced Materials Center — Environmental and Occupational Health Sciences Institute, Rutgers — The State University of New Jersey et Wyss Institute for Biologically Inspired Engineering — Harvard University, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.