Probiotic, Paraprobiotic, and Postbiotic Activities of Lactiplantibacillus plantarum KUNN19-2 Against Non-Typhoidal Salmonella Serovars
Rattachement africain : cn, th. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Non-typhoidal salmonellosis (NTS) caused by multidrug-resistant (MDR) Salmonella enterica is a significant public health concern worldwide. Probiotics offer a potential alternative to antibiotics in many infectious diseases, including NTS. However, using living bacteria raises safety concerns in clinical settings, especially in the immunocompromised host. This study compared the anti-Salmonella and immunomodulatory effects between viable (probiotics) and heat-killed (paraprobiotics) lactic acid bacteria Lactiplantibacillus plantarum KUNN19-2 (KUNN19-2), isolated from Thai-style fermented pork (Nham), against several strains of MDR Salmonella. Only viable KUNN19-2 and its cell-free supernatant directly inhibited Salmonella growth by spot-on lawn and agar well diffusion assays. A significant reduction in Salmonella numbers in the co-culture assay with viable KUNN19-2 was observed at 12–14 h after the incubation. Viable and heat-killed KUNN19-2 exhibited moderate adhesion to human colonic epithelium (T84) cells. Pretreatment with either form of KUNN19-2 enhanced macrophage (RAW264.7) phagocytic activity against Salmonella and upregulated pro-inflammatory genes (Mip-2 and Nos2) and anti-inflammatory gene (IL10) expression, with viable KUNN19-2 showing a more potent effect. Collectively, viable KUNN19-2 can directly inhibit Salmonella growth. However, viable and heat-killed KUNN19-2 can modulate gut immunity against Salmonella infection, suggesting that paraprobiotic KUNN19-2 may serve as an alternative treatment against MDR Salmonella through host immune modulation.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Probiotic, Paraprobiotic, and Postbiotic Activities of Lactiplantibacillus plantarum KUNN19-2 Against Non-Typhoidal Salmonella Serovars
- Date Crossref
- 20/02/2025
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Affiliated Hospital of Youjiang Medical University for Nationalities Key Laboratory of Basic Research and Transformation of Tumor Immunity and Infectious Diseases pays non établi dans la noticeÉtablissement de santé
-
Chiang Mai University Research Center for Veterinary Biosciences and Veterinary Public Health pays non établi dans la noticeUniversité ou école supérieure
-
Kasetsart University pays non établi dans la noticeUniversité ou école supérieure
-
Faculty of Medicine Department of Microbiology pays non établi dans la noticeUniversité ou école supérieure
-
Faculty of Veterinary Medicine pays non établi dans la noticeUniversité ou école supérieure
-
Faculty of Agro-Industry Department of Biotechnology pays non établi dans la noticeUniversité ou école supérieure
Key Laboratory of Basic Research and Transformation of Tumor Immunity and Infectious Diseases — Affiliated Hospital of Youjiang Medical University for Nationalities, Research Center for Veterinary Biosciences and Veterinary Public Health — Chiang Mai University et Kasetsart University, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.