Molecular Dissection of Symptom Determinants in Tomato Leaf Curl New Delhi Virus in Zucchini Through Mechanical Transmission
Rattachement africain : vn, kr, it. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Among begomovirus species, tomato leaf curl New Delhi virus (ToLCNDV) is significant and stands out as a mechanically transmissible bipartite begomovirus originating from the Old World. However, the mechanisms underlying the mechanical transmission of different ToLCNDV strains remain understudied, as their natural transmission occurs via insect vectors. In this study, we investigated the mechanical transmissibility of two ToLCNDVs, one from Italy and another from Pakistan, in host plants. Several cucurbit species were screened, and symptom differences between the two ToLCNDV clones were observed only in zucchini when subjected to rubbing inoculation. The Italian isolate (ToLCNDV-ES) induced typical disease symptoms such as leaf curling, yellow mosaic, and internode stunting, whereas a normal phenotype was observed in zucchini mechanically infected with ToLCNDV-In (Pakistani isolate). Subsequently, a gene-swapping experiment between the two ToLCNDVs was conducted, and ToLCNDV-ES DNA-B was identified as a crucial factor in mechanical transmission. We then constructed chimeric mutant clones based on the DNA-B sequence and assessed their ability to induce symptoms in zucchini. These results indicated that the nuclear shuttle protein is a determinant of symptom development during ToLCNDV mechanical transmission. Moreover, several defense-related host genes showed significant changes in relative expression in different ToLCNDV clones, indicating their potential role in disease symptom development through the mechanical transmission of ToLCNDV. This is the first report comparing the mechanical transmissibility of two isolates of different ToLCNDV strains from the Mediterranean region and the Indian subcontinent in the same host plant, providing new insights into the virus's pathogenicity across different geographic regions.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Molecular Dissection of Symptom Determinants in Tomato Leaf Curl New Delhi Virus in Zucchini Through Mechanical Transmission
- Date Crossref
- 20/02/2025
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Ho Chi Minh City Open University pays non établi dans la noticeUniversité ou école supérieure
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Sungkyunkwan University Department of Integrative Biotechnology pays non établi dans la noticeUniversité ou école supérieure
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Gyeongguk National University pays non établi dans la noticeUniversité ou école supérieure
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Institute for Sustainable Plant Protection pays non établi dans la noticeStructure de recherche
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National Research Council pays non établi dans la noticeOrganisation à but non lucratif
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Korea Institute of Ocean Science and Technology pays non établi dans la noticeStructure de recherche
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Faculty of Biotechnology pays non établi dans la noticeUniversité ou école supérieure
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Andong National University Department of Plant Medicals pays non établi dans la noticeUniversité ou école supérieure
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Korea Institute of Ocean Science & Technology Ecological Risk Research Department pays non établi dans la noticeStructure de recherche
Ho Chi Minh City Open University, Department of Integrative Biotechnology — Sungkyunkwan University et Gyeongguk National University, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.