Strategic Sampling of Eurasian Otter Spraints for Genetic Research in South Korea: Enhancing PCR Success and Data Accuracy
Rattachement africain : kr. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Non-invasive genetic approaches, particularly using fecal samples, are commonly used to study endangered and elusive species, as they are easy to collect with minimal permission and cause little disturbance to the subject population. However, such studies face limitations due to poor DNA yield, which affects the overall utilization of collected samples and increases data errors. Here, we evaluated the impact of sample age and collection season on the performance of DNA extracted from feces (spraints) of the Eurasian otter (Lutra lutra), a semi-aquatic apex predator in South Korean freshwater ecosystems. We found that PCR amplification success rates decreased more rapidly in summer (79.3–58.2%) compared to winter (99.2–84.8%) with extended environmental exposure. Genotyping error rates were higher in samples collected during summer, with the rate of error increase over time being significantly greater in summer than in winter. The hot and humid South Korean summer fosters microbial growth and fecal degradation, which negatively impacts DNA yield, reducing PCR amplification success and increasing genotyping errors. We recommend collecting otter feces during winter for better DNA quality. If sampling in summer is unavoidable, it is crucial to collect fresh samples, which can be facilitated by conducting frequent surveys of latrine sites.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Strategic Sampling of Eurasian Otter Spraints for Genetic Research in South Korea: Enhancing PCR Success and Data Accuracy
- Date Crossref
- 17/02/2025
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Seoul National University Comparative Medicine Disease Research Center (CDRC) pays non établi dans la noticeUniversité ou école supérieure
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Research Institute for Veterinary Science pays non établi dans la noticeStructure de recherche
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Bank of Korea pays non établi dans la noticeInstitution
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Konkuk University Department of Biological Sciences pays non établi dans la noticeUniversité ou école supérieure
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Conservation Genome Resource Bank for Korean Wildlife pays non établi dans la noticeInstitution
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College of Veterinary Medicine Research Institute for Veterinary Science pays non établi dans la noticeUniversité ou école supérieure
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College of Agriculture and Life Sciences Department of Agriculture pays non établi dans la noticeUniversité ou école supérieure
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Association of Korean Otter Conservation pays non établi dans la noticeOrganisation à but non lucratif
Comparative Medicine Disease Research Center (CDRC) — Seoul National University, Research Institute for Veterinary Science et Bank of Korea, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.