Quercetin inhibits largemouth bass rhabdovirus infection by enhancing the immune response in Micropterus salmoides

Le résumé fourni par la source

The Largemouth bass Rhabdovirus (LMBRaV) is a highly virulent pathogen that results in substantial mortality rates and economic losses within the largemouth bass ( Micropterus salmoides ) aquaculture industry. The utilization of medicinal plant extracts has emerged as a promising strategy for combating aquatic animal diseases. In this study, we aimed to evaluate the anti-LMBRaV properties effects of nine plant-derived compounds utilizing EPC cells and largemouth bass. Among them, quercetin demonstrated a notable inhibitory effect on LMBRaV replication in EPC, with a reduction of 93.6% at 20 mg/L. Quercetin exerts its anti-LMBRaV properties on cells may result from its ability to inhibit viral entry, mitigate nuclear damage, and inactivate the virus directly. In vivo experiments showed that the preventive protection rate of quercetin against LMBRaV infection in largemouth bass reached 57.7%, and decreased LMBRaV viral loads in liver, spleen and kidney. Moreover, quercetin appeared to induce the expression of several immune-related genes, including interferon (IFN), and major histocompatibility complex 1 (MHC-1), myxovirus resistance 1 (MX1), and nuclear factor erythroid 2-related factor 2 (Nrf2), suggesting its potential for immunoregulation. Furthermore, quercetin mitigated the pathological alterations in the immune organs of largemouth bass infected with LMBRaV. In summary, quercetin shows promise as an immunomodulatory agent against LMBRaV infections in largemouth bass.

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