tRNA selectivity during ribosome-associated quality control regulates the critical sterility-inducing temperature in two-line hybrid rice
Rattachement africain : cn, cz. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
The two-line hybrid rice system, a cutting-edge hybrid rice breeding technology, has greatly boosted global food security. In thermo-sensitive genic male sterile (TGMS) lines, the critical sterility-inducing temperature (CSIT; the temperature at which TGMS lines change from male fertile to complete male sterile) acts as a key threshold. We recently uncovered that thermo-sensitive genic male sterility 5 ( tms5 ), a sterile locus presenting in over 95% of TGMS lines, leads to the overaccumulation of 2′,3′-cyclic phosphate (cP)-ΔCCA-tRNAs and a deficiency of mature tRNAs, which underlies the molecular mechanism of tms5 -mediated TGMS. However, there are a few reports on the regulatory mechanism controlling CSIT. Here, we identified a suppressor of tms5 , an amino acid substitution (T552I) in the rice Rqc2 (ribosome-associated quality control 2), increases the CSIT in tms5 lines through its C-terminal alanine and threonine modification (CATylation) activity. This substitution alters tRNA selectivity, leading to the recruitment of different tRNAs to the A-site of ribosome and CATylation rate by OsRqc2 during ribosome-associated quality control (RQC), a process that rescues stalled ribosomes and degrades abnormal nascent chains during translational elongation. Further, the mutation restores the levels of mature tRNA-Ser/Ile to increase the CSIT of tms5 lines. Our findings reveal the origin of overaccumulated cP-ΔCCA-tRNAs in tms5 lines, further deepening our understanding of the regulatory network in governing CSIT of TGMS lines containing tms5.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- tRNA selectivity during ribosome-associated quality control regulates the critical sterility-inducing temperature in two-line hybrid rice
- Date Crossref
- 06/02/2025
- Éditeur
- National Academy of Sciences
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Chinese Academy of Sciences pays non établi dans la noticeOrganisme public
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Institute of Genetics and Developmental Biology pays non établi dans la noticeStructure de recherche
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University of Chinese Academy of Sciences pays non établi dans la noticeUniversité ou école supérieure
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Czech Academy of Sciences pays non établi dans la noticeStructure de recherche
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Beijing Academy of Agriculture and Forestry Sciences pays non établi dans la noticeInstitution
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Southern University of Science and Technology Institute of Plant and Food Science pays non établi dans la noticeUniversité ou école supérieure
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Ministry of Agriculture and Rural Affairs pays non établi dans la noticeOrganisme public
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Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology pays non établi dans la noticeStructure de recherche
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Yuan Longping High-tech Agriculture Co. pays non établi dans la noticeOrganisation à but non lucratif
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Key Laboratory of Rice Germplasm Enhancement in Southern China pays non établi dans la noticeStructure de recherche
Chinese Academy of Sciences, Institute of Genetics and Developmental Biology et University of Chinese Academy of Sciences, avec 7 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.