Comparative transcriptomic and metabolomics analysis of ovary in Nilaparvata lugens after trehalase inhibition
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Le résumé fourni par la source
The fecundity of Nilaparvata lugens (brown planthopper) is influenced by trehalase (TRE). To investigate the mechanism by which trehalose affects the reproduction of N. lugens, we conducted a comparative transcriptomic and metabolomic analysis of the ovaries of N. lugens following injection with dsTREs and validamycin (a TRE inhibitor). The results revealed that 844 differentially expressed genes (DEGs) were identified between the dsGFP and dsTREs injection groups, with 317 up-regulated genes and 527 down-regulated genes. Additionally, 1451 DEGs were identified between the water and validamycin injection groups, with 637 up-regulated genes and 814 down-regulated genes. The total number of DEGs identified between the two comparison groups was 236. The overlapping DEGs were implicated in various biological processes, including protein metabolism, fatty acid metabolism, AMPK signaling, mTOR signaling, insulin/insulin-like growth factor signaling (IIS), the tricarboxylic acid (TCA) cycle, oxidative phosphorylation, and the cellular process of meiosis in oocytes. These results suggest that the inhibition of TRE expression may lead to alterations in ovarian nutrient and energy metabolism by modulating glucose transport and affecting amino acid metabolic pathways. These alterations may influence the reproduction of N. lugens by modulating reproductive regulatory signals. These findings provide robust evidence supporting the mechanism through which trehalase inhibition reduces the reproductive capacity of N. lugens.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Comparative transcriptomic and metabolomics analysis of ovary in Nilaparvata lugens after trehalase inhibition
- Date Crossref
- 01/02/2025
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Hangzhou Normal University pays non établi dans la noticeUniversité ou école supérieure
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Institute of Plant Protection pays non établi dans la noticeStructure de recherche
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Chinese Academy of Agricultural Sciences pays non établi dans la noticeOrganisme public
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College of Life and Environmental Sciences pays non établi dans la noticeUniversité ou école supérieure
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State Key Laboratory for Biology of Plant Diseases and Insect Pests pays non établi dans la noticeStructure de recherche
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Zhongyuan Research Center pays non établi dans la noticeStructure de recherche
Hangzhou Normal University, Institute of Plant Protection et Chinese Academy of Agricultural Sciences, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.