Molecular Identification and Expression Analysis of nod1/2 and tbk1 in Response to Viral or Bacterial Infection in the Spotted knifejaw (Oplegnathus Punctatus)

Le résumé fourni par la source

In this study, we investigate the role of Opnod1, Opnod2 and Optbk1 genes in antiviral and antibacterial immunity of spotted knifejaw, and the expression pattern of Opnod1, Opnod2 and Optbk1 mRNA in different tissues and at different time points were validated using qRT-PCR. The results showed that the open reading frame of Opnod1 gene was 2757 bp in length and encoded 918 amino acids, the open reading frame of Opnod2 gene was 2970 bp in length and encoded 990 amino acids,while Optbk1 gene was 2172 bp in length and encoded 723 amino acids. Both Opnod1 and Opnod2 proteins have three conserved domains (CARD, NOD and LRR), and Optbk1 has a S_TKc domain. The results using real-time quantitative PCR showed that Opnod1, Opnod2 and Optbk1 genes were mainly expressed in immune-related tissues of spotted knifejaw, with the highest relative expression of Opnod1 gene in skin tissues, Opnod2 gene in gill tissues and Optbk1 gene in liver tissues. The expression of Opnod1, Opnod2 and Optbk1 genes changed significantly in the immune tissues after infection with SKIV-SD and Vibrio harveyi. In spotted knifejaw kidney cell, the expression of Opnod1, Opnod2 and Optbk1 showed up-regulation after stimulation by poly I:C and LPS in vitro. The results suggest that the NOD1/2-TBK1 signal pathway may play an important role in the resistance of the spotted knifejaw to virus and bacteria, which will provide valuable basis for resistant disease breeding of spotted knifejaw.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.