Identification of Disease-Relevant, Sex-Based Proteomic Differences in iPSC-Derived Vascular Smooth Muscle Cells
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Le résumé fourni par la source
The prevalence of cardiovascular disease varies with sex, and the impact of intrinsic sex-based differences on vasculature is not well understood. Animal models can provide important insights into some aspects of human biology; however, not all discoveries in animal systems translate well to humans. To explore the impact of chromosomal sex on proteomic phenotypes, we used iPSC-derived vascular smooth muscle cells from healthy donors of both sexes to identify sex-based proteomic differences and their possible effects on cardiovascular pathophysiology. Our analysis confirmed that differentiated cells have a proteomic profile more similar to healthy primary aortic smooth muscle cells than iPSCs. We also identified sex-based differences in iPSC-derived vascular smooth muscle cells in pathways related to ATP binding, glycogen metabolic process, and cadherin binding as well as multiple proteins relevant to cardiovascular pathophysiology and disease. Additionally, we explored the role of autosomal and sex chromosomes in protein regulation, identifying that proteins on autosomal chromosomes also show sex-based regulation that may affect the protein expression of proteins from autosomal chromosomes. This work supports the biological relevance of iPSC-derived vascular smooth muscle cells as a model for disease, and further exploration of the pathways identified here can lead to the discovery of sex-specific pharmacological targets for cardiovascular disease.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Identification of Disease-Relevant, Sex-Based Proteomic Differences in iPSC-Derived Vascular Smooth Muscle Cells
- Date Crossref
- 29/12/2024
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Cedars-Sinai Medical Center Department of Biomedical Sciences pays non établi dans la noticeÉtablissement de santé
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Cedars-Sinai Smidt Heart Institute pays non établi dans la noticeÉtablissement de santé
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University of Southern California Alfred E. Mann Department of Biomedical Engineering pays non établi dans la noticeUniversité ou école supérieure
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Advanced Clinical Biosystems Research Institute pays non établi dans la noticeStructure de recherche
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Board of Governors Regenerative Medicine Institute pays non établi dans la noticeStructure de recherche
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David and Janet Polak Foundation Stem Cell Core Laboratory iPSC Core pays non établi dans la noticeStructure de recherche
Department of Biomedical Sciences — Cedars-Sinai Medical Center, Cedars-Sinai Smidt Heart Institute et Alfred E. Mann Department of Biomedical Engineering — University of Southern California, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.