Nucleotide-binding oligomerization domain 1 (NOD1) regulates microglial activation in pseudorabies virus infection
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Le résumé fourni par la source
The primary cause of viral encephalitis (VE) is invasion of the central nervous system (CNS) by the virus, which leads to neuroinflammation and poses a significant threat to global public health. Microglia, as CNS-resident macrophages, play a crucial role in neuroinflammation and are often identified as the preferred target for the prevention or treatment of VE. In this study, we used pseudorabies virus (PRV)-induced VE in mice and pigs as a model to investigate the regulation of microglial responses during viral encephalitis and explored the mechanism of microglial activation. Cellular experiments revealed that microglial activation was accompanied by cell migration, characteristic morphological changes, phagocytosis, inflammatory cytokine production, and antigen presentation. Transcriptome analysis revealed that genes related to inflammation in PRV-infected BV2 cells were significantly enriched. The expression of the NOD1 gene in BV2 cells was significantly increased during PRV infection, after which NOD1 in BV2 cells was silenced by siRNA and overexpressed via a plasmid. NOD1 was found to be involved in the secretion of cytokines in BV2 cells by regulating the MAPK/NF-κB signalling pathway. Mouse and pig experiments have shown that NOD1 is involved in the secretion of cytokines by microglia by regulating the MAPK/NF-κB signalling pathway during PRV infection.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Nucleotide-binding oligomerization domain 1 (NOD1) regulates microglial activation in pseudorabies virus infection
- Date Crossref
- 18/12/2024
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Huazhong Agricultural University pays non établi dans la noticeUniversité ou école supérieure
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Wuhan Academy of Agricultural Sciences pays non établi dans la noticeOrganisme public
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Hubei Academy of Agricultural Sciences pays non établi dans la noticeOrganisme public
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College of Veterinary Medicine Division of Veterinary Pathology pays non établi dans la noticeUniversité ou école supérieure
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Institute of Animal Health and Veterinary Sciences pays non établi dans la noticeStructure de recherche
Huazhong Agricultural University, Wuhan Academy of Agricultural Sciences et Hubei Academy of Agricultural Sciences, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.