Bile acids in the lower airways is associated with airway microbiota changes in chronic obstructive pulmonary disease: an observational study
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Le résumé fourni par la source
BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a complex disorder with a high degree of interindividual variability. Gastrointestinal dysfunction is common in patients with COPD and has been proposed to influence the clinical progression of the disease. Using the presence of bile acid(s) (BA) in bronchoalveolar lavage (BAL) fluid as a marker of gastric aspiration, we evaluated the relationships between BAs, clinical outcomes and bacterial lung colonisation. METHODS: We used BAL specimens from a cohort of patients with COPD and healthy controls. BAs were profiled and quantified in BAL supernatants using mass spectrometry. Microbial DNA was extracted from BAL pellets and quantified using quantitative PCR. We profiled the BAL microbiota using an amplicon sequencing approach targeting the V3-V4 region of the 16S rRNA gene. RESULTS: Detection of BAs in BAL was more likely at the earliest clinical stages of COPD and was independent of the degree of airway obstruction. BAL specimens with BAs demonstrated higher bacterial biomass and lower diversity. Likewise, the odds of recovering bacterial cultures from BAL were higher if BAs were also detected. Detection of BAs in BAL was not associated with either inflammatory markers or clinical outcomes. We also observed different bacterial community types in BAL, which were associated with different clinical groups, levels of inflammatory markers and the degree of airway obstruction. CONCLUSION: Detection of BAs in BAL was associated with alterations in the airway bacterial communities. Further studies are needed to evaluate whether BAs in BAL can be used to stratify patients and predict disease progression trajectories.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Bile acids in the lower airways is associated with airway microbiota changes in chronic obstructive pulmonary disease: an observational study
- Date Crossref
- 01/12/2024
- Éditeur
- BMJ
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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The Kids Research Institute Australia pays non établi dans la noticeOrganisation à but non lucratif
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The University of Western Australia Institute of Respiratory Health and Medical School pays non établi dans la noticeUniversité ou école supérieure
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Curtin University Curtin Health Innovation Research Institute (CHIRI) pays non établi dans la noticeUniversité ou école supérieure
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Hunter Medical Research Institute pays non établi dans la noticeStructure de recherche
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University College Cork Synthesis and Solid-State Pharmaceutical Centre pays non établi dans la noticeUniversité ou école supérieure
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Fiona Stanley Hospital Department of Respiratory Medicine pays non établi dans la noticeÉtablissement de santé
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Institute for Respiratory Health Cell Biology Group pays non établi dans la noticeStructure de recherche
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Princess Margaret Hospital for Children Department of Respiratory Medicine pays non établi dans la noticeÉtablissement de santé
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Monash Health pays non établi dans la noticeÉtablissement de santé
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Monash University pays non établi dans la noticeUniversité ou école supérieure
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Wal-yan Respiratory Research Centre pays non établi dans la noticeStructure de recherche
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European Virus Bioinformatics Centre pays non établi dans la noticeInstitution
The Kids Research Institute Australia, Institute of Respiratory Health and Medical School — The University of Western Australia et Curtin Health Innovation Research Institute (CHIRI) — Curtin University, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.