Colchicine-Induced Tetraploidy in Protocorms of Aerides rosea Lodd. ex Lindl. and Paxton. and Its Identification
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Le résumé fourni par la source
Aerides rosea (Orchidaceae) boasts high ornamental value due to its pleasant aroma, foxtail spike, and elegant floral morphology. Inducing A. rosea to become tetraploid enhances horticultural traits and facilitates fertile intergeneric hybrids through crosses with other market-available tetraploid species. The experimental design involved the application of colchicine at varying concentrations—0.05%, 0.1%, and 0.2%—to a solid medium. Exposure durations were 5, 10, and 15 days, with treatments conducted under sterile conditions on 6-week-old protocorms post-germination. Results indicated that the protocorms were sensitive to colchicine concentrations exceeding 0.05%, with high concentrations leading to a mortality rate exceeding 50%. Flow cytometry (FCM) with 4′,6-diamidino-2-phenylindole (DAPI) staining confirmed a doubling of chromosome numbers in tetraploid plants (2n = 4x = 76) compared to diploid controls (2n = 2x = 38). Induction efficiency was significantly influenced by colchicine concentration and treatment duration. A 10-day treatment with 0.2% colchicine yielded a 70.00% tetraploid induction rate; however, considering protocorm survival, a 5-day treatment with 0.05% colchicine was preferable, achieving a 63.55% survival rate and a 56.67% tetraploid induction rate. Tetraploid plants exhibited distinct morphological traits, such as a more compact growth habit, thicker leaves, and increased stem and root thickness. Leaf morphology changes included larger stomata with reduced density, denser spongy mesophyll, and more pronounced venation. Tetraploids also demonstrated a 1.94-fold increase in genome size compared to diploids. The tetraploid genotypes developed in this study hold significant potential for future Aerides breeding programs.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Colchicine-Induced Tetraploidy in Protocorms of Aerides rosea Lodd. ex Lindl. and Paxton. and Its Identification
- Date Crossref
- 18/12/2024
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Beijing Forestry University pays non établi dans la noticeUniversité ou école supérieure
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Institute of Vegetables and Flowers pays non établi dans la noticeStructure de recherche
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Chinese Academy of Agricultural Sciences pays non établi dans la noticeOrganisme public
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Ministry of Agriculture and Rural Affairs pays non établi dans la noticeOrganisme public
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Key Laboratory of Biology and Genetic Improvement of Flower Crops (North China) pays non établi dans la noticeStructure de recherche
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School of Landscape Architecture pays non établi dans la noticeUniversité ou école supérieure
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State Key Laboratory of Vegetable Biobreeding pays non établi dans la noticeStructure de recherche
Beijing Forestry University, Institute of Vegetables and Flowers et Chinese Academy of Agricultural Sciences, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.