Author response: Nitric oxide modulates contrast suppression in a subset of mouse retinal ganglion cells
Rattachement africain : de, fr. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Neuromodulators have major influences on the regulation of neural circuit activity across the nervous system. Nitric oxide (NO) has been shown to be a prominent neuromodulator in many circuits and has been extensively studied in the retina. Here, it has been associated with the regulation of light adaptation, gain control, and gap junctional coupling, but its effect on the retinal output, specifically on the different types of retinal ganglion cells (RGCs), is still poorly understood. In this study, we used two-photon Ca2+imaging and multi-electrode array (MEA) recordings to measure light-evoked activity of RGCs in the ganglion cell layer in the ex vivo mouse retina. This approach allowed us to investigate the neuromodulatory effects of NO on a cell type-level. Our findings reveal that NO selectively modulates the suppression of temporal responses in a distinct subset of contrast-suppressed RGC types, increasing their activity without altering the spatial properties of their receptive fields. Given that under photopic conditions, NO release is triggered by quick changes in light levels, we propose that these RGC types signal fast contrast changes to higher visual regions. Remarkably, we found that about one-third of the RGC types, recorded using two-photon Ca2+imaging, exhibited consistent, cell type-specific adaptational response changes throughout an experiment, independent of NO. By employing a sequential-recording paradigm, we could disentangle those additional adaptational response changes from drug-induced modulations. Taken together, our research highlights the selective neuromodulatory effects of NO on RGCs and emphasizes the need of considering non-pharmacological activity changes, like adaptation, in such study designs.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Author response: Nitric oxide modulates contrast suppression in a subset of mouse retinal ganglion cells
- Date Crossref
- 13/12/2024
- Éditeur
- eLife Sciences Publications, Ltd
- Type
- peer-review
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Senckenberg Centre for Human Evolution and Palaeoenvironment pays non établi dans la noticeStructure de recherche
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University of Tübingen Werner Reichardt Centre for Integrative Neuroscience pays non établi dans la noticeUniversité ou école supérieure
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Centre National de la Recherche Scientifique pays non établi dans la noticeOrganisme public
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Institut de la Vision pays non établi dans la noticeStructure de recherche
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Inserm pays non établi dans la noticeOrganisme public
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Sorbonne Université Institut de la Vision pays non établi dans la noticeUniversité ou école supérieure
Senckenberg Centre for Human Evolution and Palaeoenvironment, Werner Reichardt Centre for Integrative Neuroscience — University of Tübingen et Centre National de la Recherche Scientifique, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.