Desiccation Stress Triggers and Exacerbates Experimental Ocular Graft-Versus-Host-Disease
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Le résumé fourni par la source
Introduction: Ocular Graft-versus-Host Disease (oGVHD) is a rapidly progressing, sight-threatening condition of the eye following allogeneic hematopoetic stem cell transplantation (aSCT). Previously we reported in two retrospective studies, adverse environmental stress (AES) such as high air flow and low humidity during the stay on the transplant unit may be a risk factor for chronic oGVHD. To investigate which pathophysiological processes may be triggered by AES in the early phase after transplantation, we performed experiments using a mouse model of oGVHD under different environmental conditions. Methods: A minor mismatch-mouse model (129S2->C57BL/6) was used where mice either remained in standard housing (>50% humidity, no air flow) or were subjected to AES (<30% humidity, constant air flow) for 18 days after bone marrow transplantation (BMT). Systemic and ocular GVHD were monitored for up to 28 days and histological, immunohistochemical and flow-cytometric analyses of ocular tissue, tear film, lacrimal gland, ocular draining-lymph nodes and spleen were performed. Results: AES led to a significantly higher systemic and oGVHD score than in animals without AES at days 7 and days 14 after BMT. Corneal staining and blepharitis remained significantly elevated after discontinuation of AES until day 28. Tear film analysis demonstrated early elevation of Tumor Necrosis Factor (TNF), whereas increase of CD8+ T-cells, higher activation of dendritic cells and early loss of Treg activity was detected in cervical lymph nodes together with an early corneal lymphangiogenesis in desiccated animals, but not in animals kept under normal housing conditions. Conclusions: These data confirm the hypothesis that AES is an independent risk factor for oGvHD. Low humidity conditions after aSCT in the mouse model worsen the severity of systemic GVHD in the mouse model, implicating that AES has not only a local effect. Therefor avoiding AES and targeting early activation of ocular surface immune mechanisms could help to reduce both oGVHD and systemic GvHD in patients undergoing aSCT.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Desiccation Stress Triggers and Exacerbates Experimental Ocular Graft-Versus-Host-Disease
- Date Crossref
- 05/11/2024
- Éditeur
- American Society of Hematology
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Cologne Department of Internal Medicine I pays non établi dans la noticeUniversité ou école supérieure
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Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases pays non établi dans la noticeStructure de recherche
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University Hospital Cologne pays non établi dans la noticeÉtablissement de santé
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Baylor College of Medicine pays non établi dans la noticeUniversité ou école supérieure
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Düsseldorf University Hospital pays non établi dans la noticeÉtablissement de santé
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Deutsche CLL Studiengruppe (DCLLSG) pays non établi dans la noticeOrganisation à but non lucratif
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Heinrich Heine University Düsseldorf pays non établi dans la noticeUniversité ou école supérieure
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Irvine pays non établi dans la noticeInstitution
Department of Internal Medicine I — University of Cologne, Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases et University Hospital Cologne, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.