Chemoattractant-Dependent Neutrophil Migration

Résumé fourni par la source

Here we developed an in vitro model system for study a dynamic process of neutrophil granulocytes migration under the influence of two main chemoattractants responsible for neutrophil transendothelial migration: leukotriene B4 (direct migration) and lipoxin A4 (reverse migration). For the first time migration in such model was studied using scanning probe microscopy method: scanning ion-conductance microscopy, which allowed to observe neutrophil’s membrane-cytoskeleton complex stiffness modulations. Thus, Young’s modulus of the neutrophil membrane-cytoskeleton complex increased during movement, while after firm adhesion to substrate it decreased. At the same time, mobility of cells adhered to solid substrate (Petri dish surface) was reduced. These neutrophil granulocytes were also more sensitive to probe impact and cell death was visualized during scanning in the system with chemoattractant. Rigidity of the cell during necrosis dying was also decreasing. Usage of the endothelial monolayer as the substrate prevented so firm adhesion of neutrophils, however their binding to the endothelial cells were strong enough to perform continuous scanning during 30 minutes. Migration in the model with endothelial monolayer was more pronounced and allowed most neutrophils to move, while polymer substrate provoked cells adhesion.

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