Enhanced antibacterial activity of Ocimum sanctum leaf extract mediated hydroxyapatite and hydroxyapatite-iron oxide nanocomposites

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Hydroxyapatite (HA) is frequently used in a variety of biomedical applications especially in orthopedics and dental because of its outstanding bioactivity and biocompatibility. On the other hand, HA lacks inherent antibacterial properties, shows poor mechanical strength, and infection issues are still one of the main causes of implant failure. Consequently, antibacterial HA may be a potential material for HA applications. For any potential uses of modified hydroxyapatite, such as in medicine or environmental protection, magnetite (Fe 3 O 4 ) may be a crucial element because it has good mechanical properties and is preferred in biomedical systems. Moreover, Fe 3 O 4 is biocompatible and basically nontoxic to humans. Therefore, the present study aims to find a novel approach that may improve the antibacterial activity of HA and HA-Fe 3 O 4 nanocomposites and provide low toxicity to humans. The synthesized HA and HA -Fe 3 O 4 nanocomposites were characterized by XRD, FTIR, SEM, EDX, and Zeta potential analyzer. Moreover, antibacterial activity tests confirmed that the utilization of Ocimum sanctum leaf extract as a reaction medium in HA and HA-Fe 3 O 4 nanocomposites synthesis improved antibacterial activity against Escherichia coli and Staphylococcus aureus . This research demonstrates the green synthesis of HA and HA-Fe 3 O 4 nanocomposites using Ocimum sanctum leaf extract by hydrothermal method, opening the door for potential applications in biomedical fields. • Hydroxyapatite and Hydroxyapatite-Iron oxide nanocomposites were synthesized by hydrothermal method using Ocimum sanctum leaf extract. • Characterization and morphological analysis were done by XRD, FTIR, SEM, EDX, and Zeta potential. • Enhanced antibacterial activity of the synthesized samples were reported through well diffusion method against Escherichia coli and Staphylococcus aureus. • Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined via resazurin aided microdilution method.

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