Characteristics and Biological Activities of a Novel Polysaccharide R1 Isolated from Rubus chingii Hu

Le résumé fourni par la source

Hu) is a Chinese herb that is rich in nutrients and has anti-inflammatory, antibacterial, antioxidant, anti-allergic, hypoglycemic, and other effects. A water-soluble polysaccharide was extracted from raspberry by using hot water extraction then purified by DEAE-Sepharose Fast Flow column chromatography. The structural characteristics of the polysaccharide (R1) are as follows: the molar ratio of the monosaccharide composition is Ara:Gal:Xyl:Glc:Man = 31.15:27.64:13.61:13.48:10.60; the molecular weight is 32,580 Da; the methylation results show that 5-Araf is the main chain and there is a presence of 3,6-Galp, 4-Xylp, and 2,3,5-Araf branches, and that terminal Araf (T-Araf) is the major telomeric sugar. It contains α and β glycosidic bonds and is highly branched, with the presence of a helical structure. In the in vitro antioxidant assay, R1 showed the highest scavenging of superoxide anion radicals at 70.38%, followed by the scavenging of DPPH radicals at 52.9% and the scavenging of hydroxyl radicals at 29.28%. In immunomodulation and anti-cancer experiments, R1 did not significantly inhibit or promote RAW264.7 cells but was able to increase the expression of anti-inflammatory cytokines in a concentration-dependent manner. It also significantly inhibited cancer cell survival. R1 enhances immunity by limiting the proliferation of cancer cells primarily through direct inhibition while promoting the secretion of pro-inflammatory cytokines. These findings reveal the potential benefits of raspberry polysaccharides and provide evidence for developing immunologically functional products from raspberry polysaccharides.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.