Application of the Sponge Model Implants in the Study of Vaccine Memory in Mice Previously Immunized with LBSap
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Background/Objectives: Considering the large number of candidates in vaccine-testing studies against different pathogens and the amount of time spent in the preclinical and clinical trials, there is a pressing need to develop an improved in vivo system to quickly screen vaccine candidates. The model of a polyester–polyurethane sponge implant provides a rapid analysis of the specific stimulus–response, allowing the study of a compartmentalized microenvironment. The sponge implant’s defined measurements were standardized as a compartment to assess the immune response triggered by the vaccinal antigen. The LBSap vaccine (composed of Leishmania braziliensis antigens associated with saponin adjuvant) was used in the sponge model to assess the antigen-specific immunological biomarker, including memory generation after initial contact with the antigen. Methods: Mice strains (Swiss, BALB/c, and C57BL/6) were previously immunized using LBSap vaccine, followed by an antigenic booster performed inside the sponge implant. The sponge implants were assessed after 72 h, and the immune response pattern was analyzed according to leukocyte immunophenotyping and cytokine production. Results: After LBSap vaccination, the innate immune response of the antigenic booster in the sponge implants demonstrated higher levels in the Ly+ neutrophils and CD11c+ dendritic cells with reduced numbers of F4/80+ macrophages. Moreover, the adaptive immune response in Swiss mice demonstrated a high CD3+CD4+ T-cell frequency, consisting of an effector memory component, in addition to a cytoxicity response (CD3+CD8+ T cells), displaying the central memory biomarker. The major cell surface biomarker in the BALB/c mice strain was related to CD3+CD4+ effector memory, while the increased CD3+CD8+ effector memory was highlighted in C57/BL6. The cytokine profile was more inflammatory in Swiss mice, with the highest levels of IL-6, TNF, IFN-g, and IL-17, while the same cytokine was observed in in C57BL/6 yet modulated by enhanced IL-10 levels. Similar to Swiss mice, BALB/c mice triggered an inflammatory environment after the antigenic booster in the sponge implant with the increased levels in the ILL-6, TNF, and IFN-g. Conclusions: The findings emphasized the impact of genetic background on the populations engaged in immune responses, suggesting that this model can be utilized to enhance and track both innate and adaptive immune responses in vaccine candidates. Consequently, these results may inform the selection of the most suitable experimental model for biomolecule testing, taking into account how the unique characteristics of each mouse strain affect the immune response dynamics.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Application of the Sponge Model Implants in the Study of Vaccine Memory in Mice Previously Immunized with LBSap
- Date Crossref
- 26/11/2024
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Universidade Federal de Ouro Preto pays non établi dans la noticeUniversité ou école supérieure
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Universidade Federal de Minas Gerais pays non établi dans la noticeUniversité ou école supérieure
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Fundação Oswaldo Cruz pays non établi dans la noticeStructure de recherche
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Hospital Israelita Albert Einstein pays non établi dans la noticeOrganisme public
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Hospital São Paulo pays non établi dans la noticeÉtablissement de santé
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Insper pays non établi dans la noticeUniversité ou école supérieure
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Universidade José do Rosário Vellano pays non établi dans la noticeUniversité ou école supérieure
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Federal University of Ouro Preto Immunopathology Laboratory pays non établi dans la noticeUniversité ou école supérieure
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Federal University of Minas Gerais Laboratory of Biology of Cellular Interactions pays non établi dans la noticeUniversité ou école supérieure
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Instituto Oswaldo Cruz (IOC) pays non établi dans la noticeStructure de recherche
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René Rachou Research Institute Integrated Biomarker Research Group pays non établi dans la noticeStructure de recherche
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Albert Einstein Israeli Institute of Education and Research pays non établi dans la noticeStructure de recherche
Universidade Federal de Ouro Preto, Universidade Federal de Minas Gerais et Fundação Oswaldo Cruz, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.