Green Synthesis and Characterization of Silver Nanoparticles from Minthostachys acris Schmidt Lebuhn (Muña) and Its Evaluation as a Bactericidal Agent Against Escherichia coli and Staphylococus aureus

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The search for new synthesis methodologies based on the principles of green chemistry has led to various studies for the production of silver nanoparticles (AgNPs) using extracts from different parts of plants. Based on this, the present study aims to carry out green synthesis (biosynthesis), characterization, and antibacterial evaluation of reduced and stabilized silver nanoparticles (AgNPs) with aqueous extracts of Minthostachys acris in a simple, ecological, and environmentally safe manner. The extraction process of the organic components is performed using two methods: immersion and the agitation of the leaves of Minthostachys acris Schmidt Lebuhn (Muña) at 0.1% for different times (0.5, 1, 3, 6, and 10 min). Compounds such as hydroxycinnamic acid derivatives, quinic, caffeic, rosmarinic acids, and flavonols present in the Muña extract facilitate the formation of AgNPs; this compounds act as a coating and stabilizing agent. The bioactive components from natural resources facilitate the formation of AgNPs, partially or completely replacing the contaminating and toxic elements present in chemical reagents. The biosynthesis is carried out at room temperature for pH 7 and 8. The synthesized AgNPs are characterized by UV-visible spectroscopy to identify the surface plasmon resonance (SPR) band, which shows an absorption peak around 419 nm and 423 nm for pH 7 and p.H 8, respectively, and Fourier-transform infrared spectroscopy (FTIR) to identify the possible biomolecules responsible for bioreduction and stabilization, with a peak at 1634 cm−1. Dynamic light scattering (DLS) shows the hydrodynamic size of the colloidal nanoparticles between 11 and 200 nm, and scanning electron microscopy (SEM) reveals monodisperse AgNPs of different morphologies, mostly nanospheres, while Laser-Induced Breakdown Spectroscopy (LIBS) demonstrates the presence of Ag in the colloidal solution. The evaluation of the bactericidal activity of the AgNPs using the disk diffusion method against Escherichia coli (E. coli) and Staphylococus aureus (S.aureus) shows that the synthesized AgNPs have effective antibacterial activity against E. coli for the extracts obtained at 6 min for both the immersion and agitation methods, respectively. The significance of this work lies in the use of bioactive components from plants to obtain AgNPs in a simple, rapid, and economical way, with potential applications in biomedical fields.

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