Electrochemical Sensor for Dopamine Detection Based on the CRISPR/Cas12 System

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Dopamine is an important neurotransmitter and biomarker that is involved in many physiological processes in the body as well as the control of the central nervous system. Therefore, it is crucial to accurately monitor dopamine concentrations in organisms in order to comprehend their biological roles and make correct clinical diagnoses. In this work, we describe the development of an aptamer sensor utilizing gold electrodes and cyclic voltammetry. Using a self-assembly approach, a single-chain sulfhydrylated dopamine-specific aptamer was immobilized on the surface of a gold electrode to successfully create the aptamer sensor. Voltammetry was used to do a thorough electrochemical characterization in order to assess the sensor’s performance. According to the findings, the created electrochemical sensor demonstrated outstanding analytical capabilities for the detection of dopamine, including a wide linear response range, a very low detection limit, high sensitivity, and great selectivity. These characteristics make the sensor a novel approach for the quick and precise detection of dopamine, and it is anticipated that clinical diagnostics and biological research will use it extensively.

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