Comparative analysis of the B cell receptor repertoire during relapse and remission in patients with multiple sclerosis
Rattachement africain : mx, jp. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Multiple sclerosis (MS) is a chronic, multifactorial, inflammatory and demyelinating disease of the central nervous system (CNS), which involves an autoimmune response against components of the myelin sheaths. Anti-B cell therapies have been proven to be successful in reducing relapses. Therefore, the study of B cells in both phases of the disease (relapse and remission) is of great importance. Here, we analyzed peripheral blood-cell BCR repertoire from 11 MS patients during a relapse phase and during remission, 6 patients with other inflammatory neurological diseases (OIND) and 10 healthy subjects (HCs), using next generation sequencing. In addition, immunoglobulins G, M, A and D were quantified in the serum of patients and controls, using ELISA. BCR repertoire of relapsing MS patients showed lower diversity, as well as a higher rate of somatic hypermutation compared to the other study groups. Within this group, the highest percentage of shared clonotypes was observed. IGHV4-32 gene was identified as a potential differential biomarker between MS and OIND, as well as IGL3-21 gene as a potential MS biomarker. On the other hand, an elevation of IgG and IgD was found in the serum of MS patients during remission, and the serum IgG was also elevated in MS patients during relapse. In conclusion, these results show the important role of B cells in the pathogenesis of the MS relapses and a new panorama on the analysis of the peripheral blood BCR repertoire to obtain diagnostic tools for MS. Furthermore, this work highlights the need of studies in diverse populations, since results reported in Caucasian populations may not coincide with the immunological course of MS patients in other latitudes, due to differences in genetic background and environmental exposures.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Comparative analysis of the B cell receptor repertoire during relapse and remission in patients with multiple sclerosis
- Date Crossref
- 01/12/2024
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional pays non établi dans la noticeStructure de recherche
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Japanese Foundation For Cancer Research Cancer Precision Medicine Center pays non établi dans la noticeOrganisation à but non lucratif
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The University of Tokyo Institute of Medical Science pays non établi dans la noticeUniversité ou école supérieure
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Aichi Cancer Center Division of Cancer Systems Biology pays non établi dans la noticeÉtablissement de santé
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Instituto Nacional de Salud Pública pays non établi dans la noticeOrganisme public
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Instituto Nacional de Neurología y Neurocirugía pays non établi dans la noticeStructure de recherche
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Department of Infectomics and Molecular Pathogenesis pays non établi dans la noticeInstitution
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National Institute of Neurology and Neurosurgery "Manuel Velasco Suarez" (INNN) Department of Neuroimmunology pays non établi dans la noticeStructure de recherche
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Clinical Laboratory of Neurodegenerative Diseases pays non établi dans la noticeStructure de recherche
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Department of Molecular Biomedicine pays non établi dans la noticeInstitution
Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Cancer Precision Medicine Center — Japanese Foundation For Cancer Research et Institute of Medical Science — The University of Tokyo, avec 7 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.