The Creation and Validation of a New High-Performance Liquid Chromatography Method for Analyzing Pyridostigmine Bromide and its Degradation Products in a Pharmaceutical Formulation

Le résumé fourni par la source

A novel, rapid, sensitive, and validated high-performance liquid chromatographic (HPLC) technique, with high selectivity for stability indication, has been employed to determine pyridostigmine bromide and its degradation products (3-hydroxy-1-methylpyidin-1-ium bromide and 3,4-dioxo-3,4-dihydropyeidin-1-ium) in a pharmaceutical formulation. Pyridostigmine bromide and its degradation products were identified using combinations of acetonitrile and 0.01M acetate buffer (pH=6)in a ratio of 5:95 (v/v), through flow rates of about 1 ml/min at room temperature, employing a diode array detector. The linear range was determined to be 27-135µg/ml. Our findings indicate that pyridostigmine bromide is unstable in alkalineconditions but more stable in acidic environments and susceptible to oxidation.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.