Occurrence of Extended-spectrum β-lactamase (ESBL) and Carbapenemase-producing Escherichia coli isolated from Childhood Diarrhoea in Yaoundé, Cameroon
Rattachement africain : Cameroun, Afrique du Sud, gb, République centrafricaine. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Extended-spectrum β-lactamase (ESBL)-producing pathogenic E. coli is a global public health issue, especially in sub-Saharan African countries such as Cameroon. It contributes to increase significantly hospital length of stay, morbidity, mortality and economic costs because of treatment failures. This study aims at determining the resistance background and virulence profiles of ESBL- E. coli isolates among childhood diarrhoea during the cholera outbreak occuring in Yaoundé, Cameroon. During a four-month periods, from March 1st to June 30th, 2023, a total of 84 stool samples were collected from 90 under five children presenting clinical signs of gastroenteritis and attending four hospitals in Yaoundé, Cameroon. Bacterial identification was done using API20E and antimicrobial susceptibility test was performed using the Kirby-Bauer disc diffusion method. After extraction, genomic DNA was subjected to conventional and multiplex polymerase chain reaction methods (PCRs) for detection of resistance and virulence genes. Statistical analysis was performed using Epi info™ (7.2.5.0). Statistical significance was considered at a p-value < 0.05. Out of 150 patients contacted, 90 patients were enrolled, 84 samples were collected, 52.38%(44/84) and 3.57%(03/84) were confirmed as extended-spectrum β-lactamase and carbapenemase-producing E. coli respectively. The risk factors were analyzed, and children who drank natural fruit juice (OR: 0.4, p-value: 0.03) were found to be significantly associated with ESBL-producing E. coli . The ESBL-producing E. coli isolates showed a high level of resistance to amoxicillin-clavulanic acid, cefotaxime, ceftazidime, cefepime, colistin, and tetracycline. The bla CTX−M was more prevalent ß-lactamase resistance gene. The tetracycline resistance genes tet(A) and tet(B) were also detected. The most important virulence genes detected were FimH (81.81%) and papA (79.54%). These findings suggest implementing routine surveillance and screening for antimicrobial resistance among children under five. Antimicrobial stewardship strategies (ASP) need to be implemented to curb the emergence and dissemination of ESBL-producing E. coli . In addition, a national surveillance program for antimicrobial resistance needs to be implemented at local and regional levels in order to reduce morbidity in Cameroon.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Occurrence of Extended-spectrum β-lactamase (ESBL) and Carbapenemase-producing Escherichia coli isolated from Childhood Diarrhoea in Yaoundé, Cameroon
- Date Crossref
- 10/10/2024
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Université de Dschang Université de Dschang, Cameroun (code pays fourni par la source)Université ou école supérieure
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Africa Health Research Institute Afrique du Sud (code pays fourni par la source)Structure de recherche
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University of KwaZulu-Natal University of KwaZulu-Natal, Afrique du Sud (code pays fourni par la source)Université ou école supérieure
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University of St Andrews pays non établi dans la noticeUniversité ou école supérieure
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Ministry of Scientific Research and Innovation Cameroun (code pays fourni par la source)Organisme public
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Université de Yaoundé I Université de Yaoundé I, Cameroun (code pays fourni par la source)Université ou école supérieure
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Centre International De Reference Chantal Biya Cameroun (code pays fourni par la source)Institution
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Mother and Child Foundation pays non établi dans la noticeOrganisation à but non lucratif
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Centre Pasteur du Cameroun Yaoundé, Cameroun (code pays fourni par la source)Structure de recherche
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Institut Pasteur de Bangui Institut Pasteur de Bangui, République centrafricaine (code pays fourni par la source)Université ou école supérieure
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University Teaching Hospital of Yaounde University Teaching Hospital of Yaounde, Cameroun (code pays fourni par la source)Établissement de santé
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Faculty of Medicine and Pharmaceutical Sciences Department of Microbiology- Haematology and Immunology University of Dschang, Cameroun (pays nommé en fin d’affiliation)Université ou école supérieure
Université de Dschang (Université de Dschang, Cameroun), Africa Health Research Institute (Afrique du Sud) et University of KwaZulu-Natal (University of KwaZulu-Natal, Afrique du Sud), avec 9 autres affiliations. Pays d’affiliation : Cameroun, Afrique du Sud, République centrafricaine.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.