Can the anaesthetic induced liquid-to-gel phase transition temperature depression be behind the molecular mechanism of general anaesthesia?
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Le résumé fourni par la source
• Anesthetic-induced α-β phase transition temperature depression of lipid membranes is studied by experiments and simulations. • Membranes containing non-anesthetics solutes are also performed; an effect similar to that with anesthetics is observed. • Any causal relation between this phase transition point depression and the phenomenon of general anesthesia is excluded. • The reasons of this lack of causality is explained by performing computer simulations. • Causal relation between general anesthesia and anesthetic-induced changes in the lateral pressure profile is demonstrated. We report differential scanning calorimetry (DSC) measurements and extensive computer simulations to determine the gel (Lβ’) to liquid crystalline (Lα) phase transition temperature of the neat 1,2-dipalmitoyl- sn -glycero-3-phosphocholine (DPPC) membrane as well as DPPC membranes containing different amount of general anaesthetics of various kinds. In addition, measurements are reported on liposomes consisting of molecules that are chemically or, at least structurally similar to certain general anaesthetics, but lack the anaesthetic effect themselves. The aim of the study is to test one of the competing hypotheses that aims at explaining the molecular mechanism of general anaesthesia by relating it to the anaesthetic-induces depression of the temperature of the aforementioned phase transition. Our results, however, do not confirm this conjecture. Thus, while diethyl ether, a well-known general anaesthetic is found not to induce any depression of the phase transition temperature, carbon tetrachloride, a molecule having no anaesthetic effect induces a larger decrease of this temperature than any general anaesthetic considered here. Furthermore, the computer simulations performed reveal that, in fact, none of the two conditions lying behind this conjecture, i.e., that anaesthetics are ideally dissolved in the Lα but do not dissolve at all in the Lβ’ phase, are satisfied. Therefore, we rule out not only a causal relation, but even a simple correlation between the membrane phase transition depression caused by dissolved molecules and the phenomenon of general anaesthesia.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Can the anaesthetic induced liquid-to-gel phase transition temperature depression be behind the molecular mechanism of general anaesthesia?
- Date Crossref
- 01/11/2024
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Friedrich-Alexander-Universität Erlangen-Nürnberg pays non établi dans la noticeUniversité ou école supérieure
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Max Planck Institute of Biochemistry pays non établi dans la noticeStructure de recherche
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Eszterhazy Karoly Catholic University Department of Chemistry pays non établi dans la noticeUniversité ou école supérieure
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Czech Academy of Sciences pays non établi dans la noticeStructure de recherche
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Institute of Materials and Environmental Chemistry pays non établi dans la noticeStructure de recherche
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HUN-REN Research Centre for Natural Sciences pays non établi dans la noticeStructure de recherche
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Eötvös Loránd University Laboratory of Interfaces and Nanosize Systems pays non établi dans la noticeUniversité ou école supérieure
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PULS Group pays non établi dans la noticeInstitution
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Max Planck Institute for Biological Intelligence pays non établi dans la noticeStructure de recherche
Friedrich-Alexander-Universität Erlangen-Nürnberg, Max Planck Institute of Biochemistry et Department of Chemistry — Eszterhazy Karoly Catholic University, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.