Detection of Streptococcus agalactiae Causing Hemorrhage and Eye Edema in Nile tilapia (Oreochromis niloticus) by Nested PCR

Le résumé fourni par la source

Abstract In Vietnam, Nile tilapia (Oreochromis niloticus), a commonly farmed fish, suffers widespread Streptococcus agalactiae infection due to water pollution in floating cage systems. Symptoms of disease include eye edema and hemorrhagic lesions, resulting in mortality rates as high as 90%. This poses significant economic and environmental challenges, impacting fish production and causing heavy financial burdens. Hence, this research focuses on employing Nested PCR for the detection of S. agalactiae on O. niloticus. Nested PCR employs two distinct primer sets for accurate identification, enabling prompt interventions and cost-effective measures to ensure sustained profitability for farmers. During the research, the external primer SA (702 bp) and internal primer PTS (200 bp) effectively amplified their specific targets in the S. agalactiae genome without non-specific binding, as evidenced by clear and localized bands in the gel electrophoresis results, and an exogenous internal control (IC) was used to control in vitro experiments. Additionally, the primers demonstrated specificity by not amplifying the genomes of Vibrio vulnificus, Yersinia enterocolitica, and Edwardsiella ictaluri. Finally, in vivo experiments controlled by an endogenous IC displayed results consistent with prior research. Therefore, Nested PCR proved effective in detecting S. agalactiae causing eye edema and hemorrhage in O. niloticus, concluding the study.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.