Protocol to generate two distinct standard-of-care murine glioblastoma models for evaluating novel combination therapies

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In cancer research, murine models play a crucial role as highly valuable preclinical tools. Here, we present a protocol to generate a murine model of glioblastoma through the direct intracranial injection of tumor cells. We describe steps for cell culture, intracranial implantation, and standard-of-care treatments. We then detail procedures for monitoring tumor growth using bioluminescent imaging. For complete details on the use and execution of this protocol, please refer to Pelizzari-Raymundo et al. 1 • Detailed steps for engineering GB cells to express luciferase for bioluminescence imaging • Generation of xenograft and syngeneic GB models via direct intracerebral injection • Comprehensive instructions for tumor resection, including video guidance for precision • Techniques for evaluating new combination therapies in preclinical glioblastoma studies Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. In cancer research, murine models play a crucial role as highly valuable preclinical tools. Here, we present a protocol to generate a murine model of glioblastoma through the direct intracranial injection of tumor cells. We describe steps for cell culture, intracranial implantation, and standard-of-care treatments. We then detail procedures for monitoring tumor growth using bioluminescent imaging.

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