A novel antigen biomarker for detection of high-level of Loa loa microfilaremia
Rattachement africain : us, Cameroun. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
BACKGROUND: Loiasis is a disease caused by the nematode Loa loa. Serious adverse events sometimes occur in people with heavy L. loa microfilaremia after ivermectin treatment. In regions of Central Africa where loiasis is endemic, this significantly impedes global elimination programs for lymphatic filariasis and onchocerciasis that use mass distribution of ivermectin. Improved diagnostic tests to identify individuals at increased risk of serious adverse events could facilitate efforts to eliminate lymphatic filariasis and onchocerciasis in this region. METHODS AND FINDINGS: We previously identified the L. loa protein Ll-Bhp-1 in loiasis patient sera. Here, we further characterize Ll-Bhp-1 and report development of an antigen capture ELISA to detect this antigen. This assay detected Ll-Bhp-1 in 74 of 116 (63.8%) loiasis patient sera. Ll-Bhp-1 levels were significantly correlated with L. loa microfilarial counts, and the sensitivity of the assay was highest for samples from people with high counts, (94% and 100% in people with ≥20,000 and ≥50,000 microfilaria per milliliter of blood, respectively). The antigen was not detected in 112 sera from people with other filarial infections, or in 34 control sera from the USA. CONCLUSIONS: This Ll-Bhp-1 antigen assay is specific for loiasis, and highly sensitive for identifying people with high L. loa microfilarial counts who are at increased risk for serious adverse events after ivermectin treatment. L. loa antigen detection has the potential to facilitate loiasis mapping efforts and programs to eliminate lymphatic filariasis and onchocerciasis in Central Africa.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- A novel antigen biomarker for detection of high-level of Loa loa microfilaremia
- Date Crossref
- 03/09/2024
- Éditeur
- Public Library of Science (PLoS)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Washington University in St. Louis pays non établi dans la noticeUniversité ou école supérieure
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James S. McDonnell Foundation pays non établi dans la noticeOrganisation à but non lucratif
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University of Buea Parasites and Vector Biology research unit (PAVBRU) University of Buea, Cameroun (code pays fourni par la source)Université ou école supérieure
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Université de Yaoundé I Université de Yaoundé I, Cameroun (code pays fourni par la source)Université ou école supérieure
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Higher Institute for Scientific and Medical Research Yaoundé, Cameroun (code pays fourni par la source)Organisation à but non lucratif
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Washington University School of Medicine Department of Medicine pays non établi dans la noticeUniversité ou école supérieure
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Research Foundation for Tropical Diseases and the Environment (REFOTDE) Buea, Cameroun (pays nommé en fin d’affiliation)Organisation à but non lucratif
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Faculty of Medicine and Biomedical Sciences Department of Public Health University of Yaoundé I, Cameroun (pays nommé en fin d’affiliation)Université ou école supérieure
Washington University in St. Louis, James S. McDonnell Foundation et Parasites and Vector Biology research unit (PAVBRU) — University of Buea (University of Buea, Cameroun), avec 5 autres affiliations. Pays d’affiliation : Cameroun.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.