Journal of Population and Therapeutics and Clinical Pharmacology

Le résumé fourni par la source

The plant Parkia speciosa Hassk. (PsH) belongs to the family Fabaceae, and it is endemic to Southeast Asia. Its seeds have been used traditionally to treat various ailments, and several investigations have been undertaken to assess its the pharmacological activity. The aim was to ascertain whether PsH, as a hydrogen sulfide donor, could prevent hypertensive nephropathy. We employed a combined in-vitro and in-vivo approach to evaluate the effect of the plant oil on isoproterenol (ISO)-induced cardiac failure in rats. The oil was prepared by hydrodistillation and the antioxidant potential was evaluated by a reducing power assay. Serum kidney biochemical parameters (serum creatinine, urea, uric acid, blood urea nitrogen), serum electrolytes (serum sodium and potassium), and antioxidant biomarkers (malondialdehyde, superoxide dismutase and, glutathione) were measured while (cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) were examined in kidney tissue by western blot. Dissected kidney sections were also examined histopathologically with eosin-hematoxylin staining and microscopy. The plant oil possessed a maximum reducing power at a concentration of 100µM. Subsequently, in-vivo findings revealed that the oil reversed ISO modified metabolic data, serum kidney biochemical parameters, serum electrolytes, and antioxidant biomarkers in renal tissues. The oil also reversed the ISO-inhibited endogenous H2S generating enzymes, CBS and CSE as disclosed by western blot. Additionally, all findings were supported by tissue histopathology. Arising from the study outcomes, PsH oil may have value as an H2S donating nutrapharmaceutical in reducing biochemical aspects of hypertensive nephropathy.

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