Genome‐Wide Identification of Pentatricopeptide Repeat (PPR) Gene Family and Multi‐Omics Analysis Provide New Insights Into the Albinism Mechanism of Kandelia obovata Propagule Leaves
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Le résumé fourni par la source
Pentatricopeptide repeat (PPR) gene family constitutes one of the largest gene families in plants, which mainly participate in RNA editing and RNA splicing of organellar RNAs, thereby affecting the organellar development. Recently, some evidence elucidated the important roles of PPR proteins in the albino process of plant leaves. However, the functions of PPR genes in the woody mangrove species have not been investigated. In this study, using a typical true mangrove Kandelia obovata, we systematically identified 298 PPR genes and characterized their general features and physicochemical properties, including evolutionary relationships, the subcellular localization, PPR motif type, the number of introns and PPR motifs, and isoelectric point, and so forth. Furthermore, we combined genome-wide association studies (GWAS) and transcriptome analysis to identify the genetic architecture and potential PPR genes associated with propagule leaves colour variations of K. obovata. As a result, we prioritized 16 PPR genes related to the albino phenotype using different strategies, including differentially expressed genes analysis and genetic diversity analysis. Further analysis discovered two genes of interest, namely Maker00002998 (PLS-type) and Maker00003187 (P-type), which were differentially expressed genes and causal genes detected by GWAS analysis. Moreover, we successfully predicted downstream target chloroplast genes (rps14, rpoC1 and rpoC2) bound by Maker00002998 PPR proteins. The experimental verification of RNA editing sites of rps14, rpoC1, and rpoC2 in our previous study and the verification of interaction between Maker00002998 and rps14 transcript using in vitro RNA pull-down assays revealed that Maker00002998 PPR protein might be involved in the post-transcriptional process of chloroplast genes. Our result provides new insights into the roles of PPR genes in the albinism mechanism of K. obovata propagule leaves.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Genome‐Wide Identification of Pentatricopeptide Repeat (PPR) Gene Family and Multi‐Omics Analysis Provide New Insights Into the Albinism Mechanism of <i>Kandelia obovata</i> Propagule Leaves
- Date Crossref
- 02/09/2024
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Xiamen University Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems pays non établi dans la noticeUniversité ou école supérieure
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Guangxi University pays non établi dans la noticeUniversité ou école supérieure
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Jiangnan University National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing pays non établi dans la noticeUniversité ou école supérieure
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College of the Environment and Ecology Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems pays non établi dans la noticeUniversité ou école supérieure
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School of Marine Sciences Guangxi Laboratory on the Study of Coral Reefs in the South China Sea pays non établi dans la noticeUniversité ou école supérieure
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School of Food Science and Technology National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing pays non établi dans la noticeUniversité ou école supérieure
Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems — Xiamen University, Guangxi University et National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing — Jiangnan University, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.