A high-cholesterol zebrafish diet promotes hypercholesterolemia and fasting-associated liver steatosis
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Le résumé fourni par la source
Zebrafish are an ideal model organism to study lipid metabolism and to elucidate the molecular underpinnings of human lipid-associated disorders. Unlike murine models, to which various standardized high lipid diets such as a high-cholesterol diet (HCD) are available, there has yet to be a uniformly adopted zebrafish HCD protocol. In this study, we have developed an improved HCD protocol and thoroughly tested its impact on zebrafish lipid deposition and lipoprotein regulation in a dose- and time-dependent manner. The diet stability, reproducibility, and fish palatability were also validated. Fish fed HCD developed hypercholesterolemia as indicated by significantly elevated ApoB-containing lipoproteins (ApoB-LPs) and increased plasma levels of cholesterol and cholesterol esters. Feeding of the HCD to larvae for 8 days produced hepatic steatosis that became more stable and sever after 1 day of fasting and was associated with an opaque liver phenotype (dark under transmitted light). Unlike larvae, adult fish fed HCD for 14 days followed by a 3-day fast did not develop a stable fatty liver phenotype, though the fish had higher ApoB-LP levels in plasma and an upregulated lipogenesis gene fasn in adipose tissue. In conclusion, our HCD zebrafish protocol represents an effective and reliable approach for studying the temporal characteristics of the physiological and biochemical responses to high levels of dietary cholesterol and provides insights into the mechanisms that may underlie fatty liver disease.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- A high-cholesterol zebrafish diet promotes hypercholesterolemia and fasting-associated liver steatosis
- Date Crossref
- 01/10/2024
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Norwegian University of Life Sciences Department of Embryology pays non établi dans la noticeUniversité ou école supérieure
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Johns Hopkins University Department of Embryology pays non établi dans la noticeUniversité ou école supérieure
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Department of Embryology pays non établi dans la noticeStructure de recherche
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University of Baltimore pays non établi dans la noticeUniversité ou école supérieure
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Johns Hopkins Hospital pays non établi dans la noticeÉtablissement de santé
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Carnegie Institution for Science Department of Embryology pays non établi dans la noticeOrganisation à but non lucratif
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Duke University Department of Molecular Genetics and Microbiology pays non établi dans la noticeUniversité ou école supérieure
Department of Embryology — Norwegian University of Life Sciences, Department of Embryology — Johns Hopkins University et Department of Embryology, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.