Implementation of a MSRE ddPCR method for the detection of methylated WIF1 and NPY genes in colorectal cancer patients
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Le résumé fourni par la source
BACKGROUND: Colorectal cancer is a worldwide leading cause of death accounting for high-rate mortality. Mutations in the epidermal growth factor receptor and RAS/MAPK pathways, as well as altered methylation genes profiles, have been described as molecular mechanisms promoting and sustaining tumour development and progression. Aberrant methylation is a well-known epigenetic mechanism involved in gene regulation; particularly several genes were reported as hypermethylated in CRC. Recently, it was shown that epigenetic alterations in genes such as neuropeptide y, proenkephalin and Wnt inhibitory factor 1 can be used as promising disease biomarkers. Almost all methods developed for the DNA methylation analysis combined next generation sequencing, conventional qRT-PCR or ddPCR with the prior DNA modification with sodium bisulfite. METHODS AND RESULTS: We implemented a ddPCR method to assess the methylation status of Wnt inhibitory factor 1 and neuropeptide y using the methylation sensitive restriction enzyme approach that does not impact on DNA quality and guarantees the discrimination of DNA methylation independent of bisulfite conversion. CONCLUSIONS: We showed that this method is robust and sensitive also allowing the monitoring of CRC disease progression when applied to circulating free DNA samples from liquid biopsies, proving to be a fast and easy to implement assay to be used for the monitoring of the methylation pattern of clinically relevant target genes.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Implementation of a MSRE ddPCR method for the detection of methylated <i>WIF1</i> and <i>NPY</i> genes in colorectal cancer patients
- Date Crossref
- 05/08/2024
- Éditeur
- SAGE Publications
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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AULSS 2 Marca Trevigiana pays non établi dans la noticeÉtablissement de santé
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Ospedale San Bortolo pays non établi dans la noticeÉtablissement de santé
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Nuovo Ospedale San Giovanni di Dio pays non établi dans la noticeÉtablissement de santé
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Histocompatibility Laboratory AULSS 2 Marca Trevigiana pays non établi dans la noticeStructure de recherche
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Laboratory of Haematology AULSS 8 Berica Ospedale San Bortolo pays non établi dans la noticeStructure de recherche
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Unit of Medical Oncology pays non établi dans la noticeInstitution
AULSS 2 Marca Trevigiana, Ospedale San Bortolo et Nuovo Ospedale San Giovanni di Dio, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.