Proteomic changes of the bovine blood plasma in response to heat stress in a tropically adapted cattle breed
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Le résumé fourni par la source
Background Identifying molecular mechanisms responsible for the response to heat stress is essential to increase production, reproduction, health, and welfare. This study aimed to identify early biological responses and potential biomarkers involved in the response to heat stress and animal’s recovery in tropically adapted beef cattle through proteomic analysis of blood plasma. Methods Blood samples were collected from 14 Caracu males during the heat stress peak (HSP) and 16 h after it (heat stress recovery—HSR) assessed based on wet bulb globe temperature index and rectal temperature. Proteome was investigated by liquid chromatography-tandem mass spectrometry from plasma samples, and the differentially regulated proteins were evaluated by functional enrichment analysis using DAVID tool. The protein-protein interaction network was evaluated by STRING tool. Results A total of 1,550 proteins were detected in both time points, of which 84 and 65 were downregulated and upregulated during HSR, respectively. Among the differentially regulated proteins with the highest absolute log-fold change values, those encoded by the GABBR1, EPHA2, DUSP5, MUC2, DGCR8, MAP2K7, ADRA1A, CXADR, TOPBP1, and NEB genes were highlighted as potential biomarkers because of their roles in response to heat stress. The functional enrichment analysis revealed that 65 Gene Ontology terms and 34 pathways were significant (P < 0.05). We highlighted those that could be associated with the response to heat stress, such as those related to the immune system, complement system, hemostasis, calcium, ECM-receptor interaction, and PI3K-Akt and MAPK signaling pathways. In addition, the protein–protein interaction network analysis revealed several complement and coagulation proteins and acute-phase proteins as important nodes based on their centrality and edges. Conclusion Identifying differentially regulated proteins and their relationship, as well as their roles in key pathways contribute to improve the knowledge of the mechanisms behind the response to heat stress in naturally adapted cattle breeds. In addition, proteins highlighted herein are potential biomarkers involved in the early response and recovery from heat stress in tropically adapted beef cattle.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Proteomic changes of the bovine blood plasma in response to heat stress in a tropically adapted cattle breed
- Date Crossref
- 01/08/2024
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Universidade de São Paulo pays non établi dans la noticeUniversité ou école supérieure
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Empresa de Pesquisa Agropecuária de Minas Gerais pays non établi dans la noticeStructure de recherche
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University of Connecticut Department of Animal Science pays non établi dans la noticeUniversité ou école supérieure
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University of Georgia Department of Animal and Dairy Science pays non établi dans la noticeUniversité ou école supérieure
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Universidade Estadual Paulista (Unesp) pays non établi dans la noticeUniversité ou école supérieure
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Beef Cattle Research Center pays non établi dans la noticeStructure de recherche
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University of Sao Paulo (USP) Department of Genetics pays non établi dans la noticeUniversité ou école supérieure
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Agricultural Research Agency of the State of Minas Gerais (EPAMIG) pays non établi dans la noticeOrganisme public
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Angus Genetics Inc. pays non établi dans la noticeEntreprise
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School of Agricultural and Veterinary Sciences Department of Animal Science pays non établi dans la noticeUniversité ou école supérieure
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Sustainable Livestock Research Center pays non établi dans la noticeStructure de recherche
Universidade de São Paulo, Empresa de Pesquisa Agropecuária de Minas Gerais et Department of Animal Science — University of Connecticut, avec 8 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.