Harnessing Ursolic Acid Metal Complexes: Boosting Anti-Inflammatory And Immunomodulatory Effects In An Arthritic Rat Model

Résumé fourni par la source

The anti-inflammatory activities of ursolic acid (UA) a natural triterpene, copper (Cu), selenium (Se), and zinc (Zn) is well documented.This study focuses on the anti-arthritic synergistic effect of novel compounds encompassing UA+Cu, UA+Se, and UA+Zn, on Freund's complete adjuvant (FCA) arthritic rat model.Lornoxicam (LX) was used as a standard drug for comparative effects.Real-time reverse transcription polymerase chain reaction (RT-PCR) was performed to evaluate mRNA expression and enzyme-linked immunosorbent assay (ELISA), was used to determine protein levels of LOX and COX-2.Furthermore, in vitro proliferation of Concanavalin A (ConA)-stimulated splenocyte was quantified using an ELISA reader.Acute toxicity of the UA+Cu, UA+Se, and UA+Zn complexes was assessed.Combination of UA with Cu, Se, Zn resulted in significantly decreased expression of NFĸB, TNF-α, TLR2, and TLR4, on the other hand IL-4, IL-10, and IL-13 expression was significantly increased.Additionally, UA complexes significantly reduced serum C-reactive protein (CRP), serum nitric oxide (NO), serum COX-2, and 5-LOX.UA+Cu, UA+Se, and UA+Zn suppressed ConA-specific splenocyte proliferation.UA+Cu, UA+Se, UA+Zn showed no hepatotoxic and nephrotoxic potential.Overall, results reflected that novel compound, UA+Cu, UA+Se, and UA+Zn have synergistic effect as compared to Cu, Se, and Zn alone, and their results were comparable with standard drug LX.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.