Co-expression of ESBL, AmpC and MBL in Pseudomonas Isolates from a Tertiary Care Hospital in Central India

Le résumé fourni par la source

Background: Pseudomonas aeruginosa infections have grown more commonplace globally as an opportunistic infection that might have unfavorable effects on hospital and community settings.The three main mechanisms of bacterial resistance to betalactam medications are Metalloβ Lactamases (MBL), AmpC β lactamases, and Extended Spectrum Beta-Lactamases (ESBLs).Methods: Pseudomonas aeruginosa samples were recognized in this investigation using motility, gram stain, colony morphology, and biochemical responses.The CLSI criteria were followed to detect ESBL production, and the Imipenem-EDTA combination disc test was used to test for MBL production.AmpC Disc Test was used to identify AmpC beta-lactamases.Results: 204(14.0%)P. aeruginosa were identified from a total of 1457 bacterial isolates.One hundred P. aeruginosa isolates were obtained from various clinical specimens.The 74 MDR isolates were more prevalent in patients receiving care indoors; coproduction of both MBL and AmpC enzymes was detected in 3% of P. aeruginosa isolates, coproduction of ESBL and MBL enzymes was detected in 20% isolates, coproduction of ESBL and AmpC enzymes was detected in 5% of isolates, coproduction of all three enzymes i.e.ESBL, AmpC β-lactamases and MBL was not detected in any of the P. aeruginosa isolates.Conclusions: It is crucial to identify the presence of ESBL, Amp C, and MBL in hospital & community isolates.The coexistence of distinct β-lactamase classes for a single bacterial strain might present difficulties in diagnosis and therapy.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.