Impaired myoblast differentiation and muscle IGF ‐1 receptor signaling pathway activation after N‐glycosylation inhibition
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Le résumé fourni par la source
The role of N-glycosylation in the myogenic process remains poorly understood. Here, we evaluated the impact of N-glycosylation inhibition by Tunicamycin (TUN) or by phosphomannomutase 2 (PMM2) gene knockdown, which encodes an enzyme essential for catalyzing an early step of the N-glycosylation pathway, on C2C12 myoblast differentiation. The effect of chronic treatment with TUN on tibialis anterior (TA) and extensor digitorum longus (EDL) muscles of WT and MLC/mIgf-1 transgenic mice, which overexpress muscle Igf-1Ea mRNA isoform, was also investigated. TUN-treated and PMM2 knockdown C2C12 cells showed reduced ConA, PHA-L, and AAL lectin binding and increased ER-stress-related gene expression (Chop and Hspa5 mRNAs and s/uXbp1 ratio) compared to controls. Myogenic markers (MyoD, myogenin, and Mrf4 mRNAs and MF20 protein) and myotube formation were reduced in both TUN-treated and PMM2 knockdown C2C12 cells. Body and TA weight of WT and MLC/mIgf-1 mice were not modified by TUN treatment, while lectin binding slightly decreased in the TA muscle of WT (ConA and AAL) and MLC/mIgf-1 (ConA) mice. The ER-stress-related gene expression did not change in the TA muscle of WT and MLC/mIgf-1 mice after TUN treatment. TUN treatment decreased myogenin mRNA and increased atrogen-1 mRNA, particularly in the TA muscle of WT mice. Finally, the IGF-1 production and IGF1R signaling pathways activation were reduced due to N-glycosylation inhibition in TA and EDL muscles. Decreased IGF1R expression was found in TUN-treated C2C12 myoblasts which was associated with lower IGF-1-induced IGF1R, AKT, and ERK1/2 phosphorylation compared to CTR cells. Chronic TUN-challenge models can help to elucidate the molecular mechanisms through which diseases associated with aberrant N-glycosylation, such as Congenital Disorders of Glycosylation (CDG), affect muscle and other tissue functions.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Impaired myoblast differentiation and muscle <scp>IGF</scp> ‐1 receptor signaling pathway activation after N‐glycosylation inhibition
- Date Crossref
- 04/07/2024
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Urbino Department of Biomolecular Sciences pays non établi dans la noticeUniversité ou école supérieure
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University of Brescia Department of Clinical and Experimental Sciences pays non établi dans la noticeUniversité ou école supérieure
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Meyer Children's Hospital Department of Neuroscience and Medical Genetics pays non établi dans la noticeÉtablissement de santé
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Istituto Pasteur DAHFMO‐Unit of Histology and Medical Embryology pays non établi dans la noticeOrganisation à but non lucratif
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University of Florence Department of NEUROFARBA pays non établi dans la noticeUniversité ou école supérieure
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University of Catania Child Neurology and Psychiatry Unit pays non établi dans la noticeUniversité ou école supérieure
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Oasi Maria SS pays non établi dans la noticeStructure de recherche
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Istituti di Ricovero e Cura a Carattere Scientifico pays non établi dans la noticeÉtablissement de santé
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San Raffaele University of Rome pays non établi dans la noticeUniversité ou école supérieure
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University of Rome La Sapienza Laboratory Affiliated to Istituto Pasteur Italia pays non établi dans la noticeUniversité ou école supérieure
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Research Unit of Rare Diseases and Neurodevelopmental Disorders Oasi Research Institute‐IRCCS Troina Italy pays non établi dans la noticeStructure de recherche
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Department of Human Sciences for the Promotion of Quality of Life University San Raffaele Rome Italy Department of Human Sciences for the Promotion of Quality of Life pays non établi dans la noticeUniversité ou école supérieure
Department of Biomolecular Sciences — University of Urbino, Department of Clinical and Experimental Sciences — University of Brescia et Department of Neuroscience and Medical Genetics — Meyer Children's Hospital, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.