Antimicrobial Use Survey and Detection of ESBL-Escherichia coli in Commercial and Medium-/Small-Scale Poultry Farms in Selected Districts of Zambia
Rattachement africain : Zambie, jp, Ghana. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Antimicrobial resistance (AMR) among Escherichia coli from food animals is a rising problem, and heavy antimicrobial use in poultry is a contributing factor. In Zambia, studies linking poultry-associated AMR and antibiotic use (AMU) are rare. This study aimed to investigate commercial and medium-/small-scale poultry farmers’ usage of antimicrobials based on a questionnaire survey in ten districts of Zambia. In addition, the study characterized extended-spectrum β-lactamase (ESBL)-producing E. coli isolates obtained from poultry in the same districts. Data regarding knowledge and usage of antimicrobials were collected from commercial and medium-/small-scale poultry farmers using a pre-tested structured questionnaire. At the same time, cloacal samples were collected and analyzed. One hundred and fifty E. coli isolates were tested for antimicrobial susceptibility using eight antibiotic classes. The isolates were further screened for ESBL production by streaking them on cefotaxime (CTX)-supplemented MacConkey agar, then subjecting them to sequencing on a NextSeq. The questionnaire survey showed that more medium-/small-scale than commercial poultry farmers used antimicrobials (OR = 7.70, 95% CI = 2.88–20.61) but less prescriptions (OR = 0.02, 95% CI = 0.00–0.08). Susceptibility testing revealed that resistance was highest to ampicillin (128/148, 86.5%) and tetracycline (101/136, 74.3%) and that the prevalence of multidrug resistance (MDR) (28/30, 93.3%) was high. Whole-genome sequencing (WGS) of eight (8/30, 26.7%) isolates with CTX Minimum Inhibitory Concentration (MIC) ≥ 4 µg/mL revealed the presence of ESBL-encoding genes blaCTX-M-14, blaCTX-M-55, and blaTEM. WGS also detected other AMR genes for quinolones, aminoglycosides, phenicols, tetracycline, macrolides, and folate-pathway antagonists. Altogether, the questionnaire survey results showed a higher proportion of AMU and lower prescription usage among medium-/small-scale farmers. In addition, our results emphasize the circulation of ESBL-producing E. coli strains with associated MDR. It is critical to educate farmers about AMR risks and to encourage responsible usage of antimicrobials. Furthermore, there is a need to strengthen regulations limiting access to antimicrobials. Finally, there is a need to establish a one health system to guide public health response.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Antimicrobial Use Survey and Detection of ESBL-Escherichia coli in Commercial and Medium-/Small-Scale Poultry Farms in Selected Districts of Zambia
- Date Crossref
- 20/05/2024
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Ministry of Agriculture and Livestock Zambie (code pays fourni par la source)Organisme public
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University of Zambia Hokudai Centre for Zoonosis Control in Zambia Lusaka, Zambie (code pays fourni par la source)Université ou école supérieure
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Zambia National Public Health Institute Lusaka, Zambie (code pays fourni par la source)Organisme public
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Hokkaido University International Institute for Zoonosis Control pays non établi dans la noticeUniversité ou école supérieure
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World Health Organization - Zambia Zambie (code pays fourni par la source)Organisme public
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University of Ghana Ghana (code pays fourni par la source)Université ou école supérieure
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Noguchi Memorial Institute for Medical Research Ghana (code pays fourni par la source)Organisation à but non lucratif
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Central Veterinary Research Institute Lusaka, Zambie (pays nommé en fin d’affiliation)Structure de recherche
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Ministry of Fisheries and Livestock Department of Veterinary Services Lusaka, Zambie (pays nommé en fin d’affiliation)Organisme public
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School of Veterinary Medicine Department of Biomedical Sciences Lusaka, Zambie (pays nommé en fin d’affiliation)Université ou école supérieure
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School of Health Sciences Department of Biomedical Sciences Lusaka, Zambie (pays nommé en fin d’affiliation)Université ou école supérieure
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Food and Agriculture Organization of the United Nations (FAO) Lusaka, Zambie (pays nommé en fin d’affiliation)Institution
Ministry of Agriculture and Livestock (Zambie), Hokudai Centre for Zoonosis Control in Zambia — University of Zambia (Lusaka, Zambie) et Zambia National Public Health Institute (Lusaka, Zambie), avec 9 autres affiliations. Pays d’affiliation : Zambie, Ghana.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.